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Cytotoxic, biochemical and genotoxic effects of biodiesel produced by different routes on ZFL cell line

dc.contributor.authorCavalcante, Dalita G. S. M.
dc.contributor.authorDa Silva, Natara D. G.
dc.contributor.authorMarcarini, Juliana Cristina
dc.contributor.authorMantovani, Mario Sergio
dc.contributor.authorMann-Morales, Maria A. [UNESP]
dc.contributor.authorMartinez, Claudia B. R.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:54:05Z
dc.date.available2015-03-18T15:54:05Z
dc.date.issued2014-09-01
dc.description.abstractTransesterification has proved to be the best option for obtaining biodiesel and, depending on the type of alcohol used in the reaction, the type of biodiesel may be methyl ester or ethyl ester. Leaking biodiesel can reach water bodies, contaminating aquatic organisms, particularly fish. The objective of this study was to determine whether the soluble fraction of biodiesel (Bd), produced by both the ethylic (BdEt) and methylic (BdMt) routes, can cause cytotoxic, biochemical and genotoxic alterations in the hepatocyte cell line of Danio rerio (ZFL). The metabolic activity of the cell was quantified by the KIT reduction method, while genotoxic damage was analyzed by the comet assay with the addition of specific endonucleases. The production of reactive oxygen species (ROS) and antioxidant/biotransformation enzymes activity also were determined. The results indicate that both Bd increased ROS production, glutathione S-transferase activity and the occurrence of DNA damage. BdMt showed higher cytotoxicity than BdEt, and also caused oxidative damage to the DNA. In general, both Bd appear to be stressors for the cells, causing cytotoxic, biochemical and genetic alterations in ZFL cells, but the type and intensity of the changes found appear to be dependent on the biodiesel production route. (C) 2014 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Londrina, Dept Ciencias Fisiol, BR-86057970 Londrina, Parana, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Biol Geral, BR-86057970 Londrina, Parana, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, Sao Paulo, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, Sao Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundacao Araucaria
dc.format.extent1117-1125
dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2014.05.008
dc.identifier.citationToxicology In Vitro. Oxford: Pergamon-elsevier Science Ltd, v. 28, n. 6, p. 1117-1125, 2014.
dc.identifier.doi10.1016/j.tiv.2014.05.008
dc.identifier.issn0887-2333
dc.identifier.urihttp://hdl.handle.net/11449/116764
dc.identifier.wosWOS:000339599300004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofToxicology In Vitro
dc.relation.ispartofjcr3.105
dc.relation.ispartofsjr0,931
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBiofuelen
dc.subjectBiotransformationen
dc.subjectComet assayen
dc.subjectIn vitro testen
dc.subjectOxidative stressen
dc.titleCytotoxic, biochemical and genotoxic effects of biodiesel produced by different routes on ZFL cell lineen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.orcid0000-0001-5268-6508[4]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.departmentBiologia - IBpt

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