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Osteopontin in Seminal Plasma and Sperm Membrane of Dogs

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dc.contributor.authorSouza, Fabiana Ferreira de [UNESP]
dc.contributor.authorChirinea, V. H. [UNESP]
dc.contributor.authorMartins, M. I. M.
dc.contributor.authorLopes, Maria Denise [UNESP]
dc.contributor.institutionUniv Franca
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2013-09-30T18:28:30Z
dc.date.accessioned2014-05-20T13:42:00Z
dc.date.available2013-09-30T18:28:30Z
dc.date.available2014-05-20T13:42:00Z
dc.date.issued2009-07-01
dc.description.abstractThe objective of this work was to describe the presence of osteopontin (OPN) in canine seminal plasma and sperm membranes. A pool of seminal plasma and sperm membrane extract from 30 dogs was used. Polyacrylamide electrophoresis gels were performed and the bands were transferred to nitrocellulose paper and Western blot was undertaken using an antibody anti-OPN. Two and 12 bands were marked in the seminal plasma (77.2 and 15.6 kDa) and sperm membrane extracts (70.6-26.6 kDa), respectively. However, from 12 marked bands in the sperm membrane extract, only three (46.4, 37.7 and 36.5 kDa) were strongly marked. We conclude that, seminal plasma and sperm membranes from dogs contain different isoforms of OPN; yet, further studies will be necessary to determine their function in this species.en
dc.description.affiliationUniv Franca, Hosp Vet, BR-14404600 Franca, SP, Brazil
dc.description.affiliationUNESP, Dept Reprod Anim & Radiol Vet, FMVZ, Botucatu, SP, Brazil
dc.description.affiliationUniversidade Estadual de Londrina (UEL), Dept Clin Vet, Londrina, Brazil
dc.description.affiliationUnespUNESP, Dept Reprod Anim & Radiol Vet, FMVZ, Botucatu, SP, Brazil
dc.format.extent283-286
dc.identifierhttp://dx.doi.org/10.1111/j.1439-0531.2009.01447.x
dc.identifier.citationReproduction In Domestic Animals. Malden: Wiley-blackwell Publishing, Inc, v. 44, p. 283-286, 2009.
dc.identifier.doi10.1111/j.1439-0531.2009.01447.x
dc.identifier.issn0936-6768
dc.identifier.lattes6666129914663018
dc.identifier.urihttp://hdl.handle.net/11449/14580
dc.identifier.wosWOS:000267756400060
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofReproduction in Domestic Animals
dc.relation.ispartofjcr1.422
dc.relation.ispartofsjr0,594
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleOsteopontin in Seminal Plasma and Sperm Membrane of Dogsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
dspace.entity.typePublication
unesp.author.lattes6666129914663018
unesp.author.orcid0000-0003-4721-1801[1]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

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Botucatu - FMVZ - Faculdade de Medicina Veterinária e Zootecnia