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Aptamer-based Biosensor Developed to Monitor MUC1 Released by Prostate Cancer Cells

dc.contributor.authorKarpik, Agnieszka E.
dc.contributor.authorCrulhas, Bruno P. [UNESP]
dc.contributor.authorRodrigues, Carolina B. [UNESP]
dc.contributor.authorCastro, Gustavo R. [UNESP]
dc.contributor.authorPedrosa, Valber A. [UNESP]
dc.contributor.institutionInstitute of Materials Science and Engineering
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:13:00Z
dc.date.available2018-12-11T17:13:00Z
dc.date.issued2017-10-01
dc.description.abstractElectrochemical aptasensors can detect cancer biomarkers such as mucin 1 (MUC1) to provide point-of-care diagnosis that is low-cost, specific and sensitive. Herein, a DNA hairpin containing MUC1 aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. The fabrication process was characterized by scanning electron microscopy, X-ray spectroscopy analysis and electrochemistry techniques. The results evidenced a stable and sensitivity sensor presenting wide linear detection range (0.65–110 ng/mL). Therefore, it was able to precisely detect MUC1 production patterns in normal (RWPE-1) and prostate cancer cells (LNCaP and PC3). The biosensor has ability to detect MUC1 in complex samples being an efficient and useful platform for cancer diagnosing in early stages and for physiological applications such as cancer treatment monitoring.en
dc.description.affiliationFaculty of Mechanical Engineering Institute of Materials Science and Engineering
dc.description.affiliationDepartment of Chemistry and Biochemistry Institute of Bioscience UNESP
dc.description.affiliationUnespDepartment of Chemistry and Biochemistry Institute of Bioscience UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2016/15919-8
dc.format.extent2246-2253
dc.identifierhttp://dx.doi.org/10.1002/elan.201700318
dc.identifier.citationElectroanalysis, v. 29, n. 10, p. 2246-2253, 2017.
dc.identifier.doi10.1002/elan.201700318
dc.identifier.issn1521-4109
dc.identifier.issn1040-0397
dc.identifier.scopus2-s2.0-85021454092
dc.identifier.urihttp://hdl.handle.net/11449/174821
dc.language.isoeng
dc.relation.ispartofElectroanalysis
dc.relation.ispartofsjr0,692
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restritopt
dc.sourceScopus
dc.subjectaptamers
dc.subjectbiomarkers
dc.subjectcancer
dc.subjectElectrochemical detection
dc.subjectprotein monitoring
dc.titleAptamer-based Biosensor Developed to Monitor MUC1 Released by Prostate Cancer Cellsen
dc.typeArtigopt
dspace.entity.typePublication
relation.isOrgUnitOfPublicationbc74a1ce-4c4c-4dad-8378-83962d76c4fd
relation.isOrgUnitOfPublication.latestForDiscoverybc74a1ce-4c4c-4dad-8378-83962d76c4fd
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.departmentBioquímica e Tecnologia - IQpt

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