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Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats

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dc.contributor.authorCosta, V. M. [UNESP]
dc.contributor.authorLangoni, Hélio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:39:59Z
dc.date.available2014-05-20T13:39:59Z
dc.date.issued2010-01-01
dc.description.abstractThe milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.en
dc.description.affiliationSão Paulo State Univ, UNESP,Fac Med Vet & Zootecnia, Univ Estadual Paulista,Dept Higiene Vet & Saúde P, Zoonosis Res Ctr,NUPEZO,Vet Med & Anim Husb Sch, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP,Fac Med Vet & Zootecnia, Univ Estadual Paulista,Dept Higiene Vet & Saúde P, Zoonosis Res Ctr,NUPEZO,Vet Med & Anim Husb Sch, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFelipe Gazza Romao and Taticia Ykeda
dc.description.sponsorshipIdFAPESP: 05/03017-5
dc.description.sponsorshipIdFAPESP: 06/02860-3
dc.description.sponsorshipIdCNPq: 471.914/2006-1
dc.format.extent368-374
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992010000200016
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 2, p. 368-374, 2010.
dc.identifier.fileS1678-91992010000200016-en.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes5326072118518067
dc.identifier.scieloS1678-91992010000200016
dc.identifier.urihttp://hdl.handle.net/11449/13887
dc.identifier.wosWOS:000278873100016
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectToxoplasma gondiien
dc.subjectparasite in milken
dc.subjectfemale ratsen
dc.subjectPCRen
dc.subjectbioassayen
dc.titleDetection of Toxoplasma gondii in the milk of experimentally infected Wistar female ratsen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
dspace.entity.typePublication
unesp.author.lattes5326072118518067
unesp.author.orcid0000-0001-5127-0762[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentHigiene Veterinária e Saúde Pública - FMVZpt

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Botucatu - FMVZ - Faculdade de Medicina Veterinária e Zootecnia