Publicação: Absorbance detector for high performance liquid chromatography based on a deep-UV light-emitting diode at 235 nm
Carregando...
Arquivos
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
In this communication, we describe a flow-through optical absorption detector for HPLC using for the first time a deep-UV light-emitting diode with an emission band at 235 nm as light source. The detector is also comprised of a UV-sensitive photodiode positioned to enable measurement of radiation through a flow-through cuvette with round aperture of 1 mm diameter and optical path length of 10 mm, and a second one positioned as reference photodiode; a beam splitter and a power supply. The absorbance was measured and related to the analyte concentration by emulating the Lambert-Beer law with a log-ratio amplifier circuitry. This detector showed noise levels of 0.30 mAU, which is comparable with our previous LED-based detectors employing LEDs at 280 and 255 nm. The detector was coupled to a HPLC system and successfully evaluated for the determination of the anti-diabetic drugs pioglitazone and glimepiride in an isocratic separation and the benzodiazepines flurazepam, oxazepam and clobazam in a gradient elution. Good linearities (r > 0.99), a precision better than 0.85% and limits of detection at sub-ppm levels were achieved.
Descrição
Palavras-chave
Absorbance detection, Deep-UV, HPLC, Light-emitting diode
Idioma
Inglês
Como citar
Journal of Chromatography A, v. 1512, p. 143-146.
