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Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)

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dc.contributor.authorSouza, Thais T. S.
dc.contributor.authorBezerra, Maria J. B.
dc.contributor.authorvan Tilburg, Mauricio F.
dc.contributor.authorNagano, Celso S.
dc.contributor.authorRola, Luciana D. [UNESP]
dc.contributor.authorDuarte, Jose M. B. [UNESP]
dc.contributor.authorMelo, Luciana M.
dc.contributor.authorMoura, Arlindo A.
dc.contributor.authorFreitas, Vicente J. F.
dc.contributor.institutionUniv Estadual Ceara
dc.contributor.institutionUniv Fed Ceara
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Ctr Fametro
dc.date.accessioned2021-06-25T11:23:13Z
dc.date.available2021-06-25T11:23:13Z
dc.date.issued2020-04-01
dc.description.abstractThe aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (<= 3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P> 0.05) was observed between small/medium and large follicles: 49.2 +/- 22.8 and 56.7 +/- 27.4 mu g/mu l, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P> 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer.en
dc.description.affiliationUniv Estadual Ceara, Fac Vet, Av Dr Silas Munguba 1700, BR-60714903 Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Ceara, Dept Anim Sci, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Estadual Ceara, Hlth Sci Ctr, Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Ceara, Lab Mass Spectrometry, Fortaleza, Ceara, Brazil
dc.description.affiliationState Univ Sao Paulo, Dept Anim Sci, Jaboticabal, Brazil
dc.description.affiliationUniv Ctr Fametro, Mol Genet Res Unit, Fortaleza, Ceara, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Dept Anim Sci, Jaboticabal, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship(FUNCAP)
dc.description.sponsorshipIdCNPq: 461330/2014-8
dc.description.sponsorshipId(FUNCAP): CI3-0093-0005580100/14
dc.format.extent170-173
dc.identifierhttp://dx.doi.org/10.1017/S0967199419000741
dc.identifier.citationZygote. New York: Cambridge Univ Press, v. 28, n. 2, p. 170-173, 2020.
dc.identifier.doi10.1017/S0967199419000741
dc.identifier.issn0967-1994
dc.identifier.urihttp://hdl.handle.net/11449/208868
dc.identifier.wosWOS:000578224800012
dc.language.isoeng
dc.publisherCambridge Univ Press
dc.relation.ispartofZygote
dc.sourceWeb of Science
dc.subjectDeer
dc.subjectElectrophoresis
dc.subjectMS
dc.subjectSDS-PAGE
dc.titleProtein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)en
dc.typeArtigopt
dcterms.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dcterms.rightsHolderCambridge Univ Press
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias