Sex determination of murine and bovine embryos using cytotoxicity and immunofluorescence assays
| dc.contributor.author | Hossepian de Lima, V. F M | |
| dc.contributor.author | Moreira-Filho, C. A. | |
| dc.contributor.author | De Bem, A. R. | |
| dc.contributor.author | Jorge, W. [UNESP] | |
| dc.contributor.institution | Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) | |
| dc.contributor.institution | Universidade de São Paulo (USP) | |
| dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
| dc.date.accessioned | 2014-05-27T11:17:53Z | |
| dc.date.available | 2014-05-27T11:17:53Z | |
| dc.date.issued | 1993-06-01 | |
| dc.description.abstract | Murine and bovine embryos were sexed by using polyclonal and monoclonal anti-H-Y antibodies in cytotoxic and immunofluorescence assays. The specificity of 3 different sources of antibodies are compared. Sexing was accomplished by complement-mediated cytotoxicity or by H-Y antigen detection using a anti-mouse Ig fluorescein conjugate. Cytogenetic ascertainment for correct sex identification yielded a score of about 85% in both species, while the immunofluorescence assay yielded a score of 83%. © 1993. | en |
| dc.description.affiliation | Embrapa-Cenargen, Caixa Postal 02372, CEP 70770 Brasília, DF | |
| dc.description.affiliation | Departamento de Imunologia ICB USP São Paulo, SP | |
| dc.description.affiliation | Departamento de Genética UNESP Botucatu, SP | |
| dc.description.affiliationUnesp | Departamento de Genética UNESP Botucatu, SP | |
| dc.format.extent | 1343-1352 | |
| dc.identifier | http://dx.doi.org/10.1016/0093-691X(93)90236-X | |
| dc.identifier.citation | Theriogenology, v. 39, n. 6, p. 1343-1352, 1993. | |
| dc.identifier.doi | 10.1016/0093-691X(93)90236-X | |
| dc.identifier.issn | 0093-691X | |
| dc.identifier.scopus | 2-s2.0-38249001637 | |
| dc.identifier.uri | http://hdl.handle.net/11449/64377 | |
| dc.identifier.wos | WOS:A1993LF25800013 | |
| dc.language.iso | eng | |
| dc.relation.ispartof | Theriogenology | |
| dc.relation.ispartofjcr | 2.136 | |
| dc.rights.accessRights | Acesso restrito | pt |
| dc.source | Scopus | |
| dc.subject | anti-H-Y antibodies | |
| dc.subject | embryo | |
| dc.subject | immunological assays | |
| dc.subject | sexing | |
| dc.title | Sex determination of murine and bovine embryos using cytotoxicity and immunofluorescence assays | en |
| dc.type | Artigo | pt |
| dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dspace.entity.type | Publication | |
| relation.isOrgUnitOfPublication | ab63624f-c491-4ac7-bd2c-767f17ac838d | |
| relation.isOrgUnitOfPublication.latestForDiscovery | ab63624f-c491-4ac7-bd2c-767f17ac838d | |
| unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatu | pt |
| unesp.department | Genética - IBB | pt |