Veterinary disease-oriented Biobanking for biomolecular analysis based on frozen tumor biopsies, cell culture and forensic tissues1

Abstract

Collecting and analyzing biological specimens leads to unprecedented opportunities for assessing and understanding the complex networks of interactions between biomolecules, and the functional and clinical consequences of their alterations. In this context, the Tissue Bank plays a key role in life science research and development, making its implementation in veterinary medicine essential for developing new research, especially in the forensic and tumor fields. In total, 52 tissues were collected, 15 forensic samples, 25 neoplasms, and 12 cell cultures. The storage, freezing, data management protocols, and the quality of these samples were analyzed. In the same way, the quality of the RNA and DNA in the short and long term was evaluated. The quality of the genetic material was confirmed by polymerase chain reaction. For data comparisons, non-parametric tests were used, with a significance level of p<0.05. The measures adopted in the Tissue Bank for obtaining and handling the samples and for controlling and guaranteeing the quality were considered adequate, as they enabled optimal preservation of the integrity of the genetic material, a relevant factor for the use of tumor samples and forensic samples stored in the Biobank, thus contributing to future molecular research in the area of pathology and investigations of crimes against fauna.