Publicação:
Demonstration of the cellular viability and safety of Enterococcus faecium CRL 183 in long-term experiments

dc.contributor.authorCano, Veridiana P. S.
dc.contributor.authorValentini, Sandro Roberto [UNESP]
dc.contributor.authorSivieri, Katia [UNESP]
dc.contributor.authorRossi, Elizeu Antonio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:26:05Z
dc.date.available2014-05-20T15:26:05Z
dc.date.issued2007-01-01
dc.description.abstractEnterococcus faecium CRL 183, a strain isolated from NSLAB cheese starter, has been the focus of much research on its potential probiotic capacity, although its survival through the gastrointestinal tract has not been demonstrated so far. In order to determine the capacity of E. faecium CRL 183 to survive such conditions, this strain was administered daily to rats for 30 weeks. The experimental animals were divided into Group I: those that did not receive E. faecium, Group II: those that received a pure culture of E. faecium CRL 183 and Group III: animals that received E. faecium CRL 183 in the form of a fermented soy-based product. Faecal samples were collected at the beginning and at the 50%, 75% and 100% stages of the experimental period. Isolation and counts of Enterococcus were carried out on KF selective media. To distinguish the various Enterococcus species in the faeces, biochemical (API Strep 20) and molecular (PCR) tests were performed. Initially, E. faecium was absent from the intestinal flora of the rats; however, after 15 weeks of administration, E. faecium could be recovered from the faeces of Groups II and III, demonstrating that E. faecium CRL 183 was able to survive gastrointestinal transit under the study conditions. This is further evidence of the probiotic qualities of this strain. The safety of the strain was also investigated with regard to body weight and serum biochemical analysis.en
dc.description.affiliationUNESP, Dept Food Nutr, Araraquara, SP, Brazil
dc.description.affiliationUNESP, Dept Biol, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Food Nutr, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol, Araraquara, SP, Brazil
dc.format.extent59-69
dc.identifierhttp://dx.doi.org/10.1051/lait:2007002
dc.identifier.citationLait. Les Ulis Cedex A: Edp Sciences S A, v. 87, n. 1, p. 59-69, 2007.
dc.identifier.doi10.1051/lait:2007002
dc.identifier.issn0023-7302
dc.identifier.lattes5333250355049814
dc.identifier.lattes3242858535763793
dc.identifier.urihttp://hdl.handle.net/11449/36325
dc.identifier.wosWOS:000246473100004
dc.language.isoeng
dc.publisherEdp Sciences S A
dc.relation.ispartofLait
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectprobioticpt
dc.subjectEnterococcus faeciumpt
dc.subjectratpt
dc.subjectsurvivalpt
dc.titleDemonstration of the cellular viability and safety of Enterococcus faecium CRL 183 in long-term experimentsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderEdp Sciences S A
dspace.entity.typePublication
unesp.author.lattes5333250355049814
unesp.author.lattes3242858535763793
unesp.author.orcid0000-0001-8632-1478[3]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAlimentos e Nutrição - FCFpt

Arquivos

Licença do Pacote

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: