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Lipopolysaccharide and lipotheicoic acid differentially modulate epididymal cytokine and chemokine profiles and sperm parameters in experimental acute epididymitis

dc.contributor.authorSilva, Erick J. R. [UNESP]
dc.contributor.authorRibeiro, Camilla M.
dc.contributor.authorMirim, Andre F. M.
dc.contributor.authorSilva, Alan A. S. [UNESP]
dc.contributor.authorRomano, Renata M.
dc.contributor.authorHallak, Jorge
dc.contributor.authorAvellar, Maria Christina W.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSci & Innovat Ctr Androl
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionState Univ Centro Oeste
dc.date.accessioned2018-11-26T17:44:38Z
dc.date.available2018-11-26T17:44:38Z
dc.date.issued2018-01-08
dc.description.abstractBacterial infections are the most prevalent etiological factors of epididymitis, a commonly diagnosed inflammatory disease in the investigation of male infertility factors. The influence of early pathogenic mechanisms at play during bacterial epididymitis on reproductive outcomes is little understood. We report here that experimental epididymitis induced in rats by Gram-negative (LPS) and Gram-positive (LTA) bacterial products resulted in differential patterns of acute inflammation in the cauda epididymis. LPS elicited a strong inflammatory reaction, as reflected by upregulation of levels of mRNA for seven inflammatory mediators (Il1b, Tnf, Il6, Ifng, Il10, Nos2 and Nfkbia), and tissue concentration of six cytokines/chemokines (IL1A, IL1B, IL6, IL10, CXCL2 and CCL2) within the first 24 h post-treatment. Conversely, LTA induced downregulation of one (Nfkbia) and upregulation of six (Il1b, Il6, Nos2, Il4 Il10 and Ptgs1) inflammatory gene transcripts, whereas increased the tissue concentration of three cytokines/chemokines (IL10, CXCL2 and CCL2). The stronger acute inflammatory response induced by LPS correlated with a reduction of epididymal sperm count and transit time that occurred at 1, 7, and 15 days post-treatment. Our study provides evidence that early epididymal inflammatory signaling events to bacterial activators of innate immunity may contribute to the detrimental effects of epididymitis upon male fertility.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Sect Expt Endocrinol, Dept Pharmacol, BR-04044020 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Pharmacol, Inst Biosci Botucatu, Botucatu, SP, Brazil
dc.description.affiliationSci & Innovat Ctr Androl, Androsci, BR-03178200 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Med Sch, Hosp Clin, Reprod Toxicol Unity,Dept Pathol, BR-01246903 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Med Sch, Hosp Clin, Div Urol, BR-01246903 Sao Paulo, SP, Brazil
dc.description.affiliationState Univ Centro Oeste, Dept Pharm, BR-85040080 Guarapuava, PR, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Pharmacol, Inst Biosci Botucatu, Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipPro-Reitoria de Pesquisa/UNESP
dc.description.sponsorshipIdCNPq: 401718/2012-3
dc.description.sponsorshipIdCNPq: 479546/2013-4
dc.description.sponsorshipIdCNPq: 455450/2014-5
dc.description.sponsorshipIdCNPq: 308349/2010-5
dc.description.sponsorshipIdFAPESP: 2010/52711-0
dc.description.sponsorshipIdFAPESP: 2015/08227-0
dc.description.sponsorshipIdPro-Reitoria de Pesquisa/UNESP: 557
dc.format.extent12
dc.identifierhttp://dx.doi.org/10.1038/s41598-017-17944-4
dc.identifier.citationScientific Reports. London: Nature Publishing Group, v. 8, 12 p., 2018.
dc.identifier.doi10.1038/s41598-017-17944-4
dc.identifier.fileWOS000419442500040.pdf
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11449/163701
dc.identifier.wosWOS:000419442500040
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.relation.ispartofsjr1,533
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleLipopolysaccharide and lipotheicoic acid differentially modulate epididymal cytokine and chemokine profiles and sperm parameters in experimental acute epididymitisen
dc.typeArtigo
dcterms.rightsHolderNature Publishing Group
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentFarmacologia - IBBpt

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