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Effect of avocado/soybean unsaponifiables on ligature-induced bone loss and bone repair after ligature removal in rats

dc.contributor.authorOliveira, G. J. P. L. [UNESP]
dc.contributor.authorPaula, L. G. F. [UNESP]
dc.contributor.authorSouza, J. A. C. [UNESP]
dc.contributor.authorSpin-Neto, R.
dc.contributor.authorStavropoulos, A.
dc.contributor.authorMarcantonio, R. A. C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAarhus University
dc.contributor.institutionMalmö University
dc.date.accessioned2015-12-07T15:36:48Z
dc.date.available2015-12-07T15:36:48Z
dc.date.issued2015-08-06
dc.description.abstractThe aim of this study was to evaluate the effects of administration of avocado/soybean unsaponifiable (ASU), a drug that is commonly used in the treatment of rheumatoid arthritis, on ligature-induced bone loss and bone repair after ligature removal in rats. Eighty-four rats were randomly assigned to four groups of equal size and received a daily gavage of either sterile saline [control (CTR)] or ASU (0.6 mg/kg), starting 7 d before (ASU/-7), on the day of (ASU/0) or 7 d after (ASU/+7) periodontitis induction. Periodontitis was induced by placing silk ligatures into the gingival sulcus of the second maxillary molars for 7 d; after 7 d, the ligatures were removed. Seven rats from each group were sacrificed, 7, 15 or 30 d after ligature removal. Bone resorption was evaluated by histomorphometry and micro-computed tomography (micro-CT). Immunohistochemistry was used to evaluate the expression of TRAP, RANKL and alkaline phosphatase (ALP), and quantitative PCR (qPCR) was used to evaluate the levels of interleukin-1beta (Il1β), tumor necrosis factor alpha (Tnfα), interleukin-6 (Il-6), Rankl and Alp. Statistical analysis was performed using the Shapiro-Wilk test, ANOVA and Tukey's test for normal data, and using the Kruskall-Wallis and Dunnet's tests for non-normal data (p < 0.05). Histomorphometry and micro-CT analysis showed greater bone resorption in the CTR group than in the ASU/0 (15 d) and ASU/+7 (7 and 15 d) groups. The CTR group also presented with a higher expression of TRAP (15 and 30 d) and RANKL (7 and 15 d) compared with ASU/0 and ASU/+7 groups. Similarly, qPCR analysis showed higher levels of Rankl and Il1β mRNAs, and lower levels of Alp mRNA, in the CTR group compared with all other groups (for all periods). ASU exhibited a positive effect on bone repair following ligature-induced periodontitis in rats.en
dc.description.affiliationSection of Periodontology, Department of Diagnosis and Surgery, Araraquara School of Dentistry, University Estadual Paulista - UNESP, Araraquara, SP, Brazil.
dc.description.affiliationSection of Oral Radiology, Department of Dentistry, Aarhus University, Aarhus, Denmark.
dc.description.affiliationDepartment of Periodontology, Faculty of Odontology, Malmö University, Malmö, Sweden.
dc.description.affiliationUnespSection of Periodontology, Department of Diagnosis and Surgery, Araraquara School of Dentistry, University Estadual Paulista - UNESP, Araraquara, SP, Brazil.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2010/13029-9
dc.description.sponsorshipIdFAPESP: 2009/17395-2
dc.format.extent1-10
dc.identifierhttp://dx.doi.org/10.1111/jre.12312
dc.identifier.citationJournal Of Periodontal Research, p. 1-10, 2015.
dc.identifier.doi10.1111/jre.12312
dc.identifier.issn1600-0765
dc.identifier.lattes3534044399884035
dc.identifier.pubmed26248485
dc.identifier.urihttp://hdl.handle.net/11449/131516
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal Of Periodontal Research
dc.relation.ispartofsjr0,927
dc.rights.accessRightsAcesso restritopt
dc.sourcePubMed
dc.subjectAnimal studiesen
dc.subjectAvocado/soybean unsaponifiablesen
dc.subjectHost modulationen
dc.subjectPeriodontitisen
dc.titleEffect of avocado/soybean unsaponifiables on ligature-induced bone loss and bone repair after ligature removal in ratsen
dc.typeArtigopt
dcterms.rightsHolderWiley-Blackwell
dspace.entity.typePublication
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
relation.isOrgUnitOfPublication.latestForDiscoveryca4c0298-cd82-48ee-a9c8-c97704bac2b0
unesp.author.lattes3534044399884035
unesp.author.orcid0000-0002-5052-7439[6]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentDiagnóstico e Cirurgia - FOARpt

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