New molecular features of cowpea bean (Vigna unguiculata, l. Walp) beta-vignin
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Taylor & Francis Ltd
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Cowpea seed -vignin, a vicilin-like globulin, proved to exert various health favourable effects, including blood cholesterol reduction in animal models. The need of a simple scalable enrichment procedure for further studies for tailored applications of this seed protein is crucial. A chromatography-independent fractionation method allowing to obtain a protein preparation with a high degree of homogeneity was used. Further purification was pursued to deep the molecular characterisation of -vignin. The results showed: (i) differing glycosylation patterns of the two constituent polypeptides, in agreement with amino acid sequence features; (ii) the seed accumulation of a gene product never identified before; (iii) metal binding capacity of native protein, a property observed only in few other legume seed vicilins.
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Vigna ssp, vicilin proteins, beta-vignin, glycosylation, metal ion binding
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Inglês
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Bioscience Biotechnology And Biochemistry. Abingdon: Taylor & Francis Ltd, v. 82, n. 2, p. 285-291, 2018.
