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RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes

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dc.contributor.authorDi Mauro, Antonio Orlando [UNESP]
dc.contributor.authorDi Mauro, Daniela Cristina [UNESP]
dc.contributor.authorCapeloto, Andrea [UNESP]
dc.contributor.authorArriel, Nair Helena de Castro
dc.contributor.authorCosta, Marcelo Marchi [UNESP]
dc.contributor.authorTrevisoli, Sandra Helena Uneda
dc.contributor.authorDi Mauro, Sonia Marli Zingaretti [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionAgência Paulista de Tecnologia dos Agronegócios (APTA)
dc.date.accessioned2014-05-27T11:22:03Z
dc.date.available2014-05-27T11:22:03Z
dc.date.issued2006-12-01
dc.description.abstractThe Random Amplified Polymorphic DNA (RAPD) technique is powerful for DNA polymorphism determinations and is widely used in research involving different organisms, but it is known that RAPD can be affected by many factors that may result in false positive bands and non-reproducible assays. In this study, we analyzed the effect of several factors such as DNA template, primer and Taq DNA polymerase concentrations to optimize and standardize the RAPD technique for further genetic studies with Citrulus lanattus and Sesamum indicum L. The best combination of DNA, Taq DNA polymerase enzyme and primer concentrations in RAPD amplification procedures for sesame and watermelon genotypes was established.en
dc.description.affiliationDepartamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SP
dc.description.affiliationDepartamento de Tecnologia UNESP
dc.description.affiliationEmbrapa Algodão, Rua Oswaldo Cruz, 1143, Centenário, 58.107-720, Campina Grande, PB
dc.description.affiliationAPTA Regional Centro Leste, C. P. 271, 14.001-970, Ribeirão Preto, SP
dc.description.affiliationUnespDepartamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SP
dc.description.affiliationUnespDepartamento de Tecnologia UNESP
dc.format.extent167-170
dc.identifierhttp://dx.doi.org/10.12702/1984-7033.v06n02a08
dc.identifier.citationCrop Breeding and Applied Biotechnology, v. 6, n. 2, p. 167-170, 2006.
dc.identifier.doi10.12702/1984-7033.v06n02a08
dc.identifier.file2-s2.0-41149127363.pdf
dc.identifier.issn1518-7853
dc.identifier.lattes1275652518822095
dc.identifier.scopus2-s2.0-41149127363
dc.identifier.urihttp://hdl.handle.net/11449/69276
dc.language.isoeng
dc.relation.ispartofCrop Breeding and Applied Biotechnology
dc.relation.ispartofsjr0,609
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectConditions, watermelon, sesame
dc.subjectDNA amplification
dc.subjectCitrullus lanatus var. lanatus
dc.subjectSesamum indicum
dc.titleRAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypesen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/cbab/paboutj.htm
dspace.entity.typePublication
unesp.author.lattes1275652518822095[1]
unesp.author.orcid0000-0003-1662-5745[1]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentProdução Vegetal - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias