Logotipo do repositório
 

Publicação:
Overexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stress

Página do item simplificado
dc.contributor.authorBrandalise, M.
dc.contributor.authorMaia, Ivan de Godoy [UNESP]
dc.contributor.authorBorecky, J.
dc.contributor.authorVercesi, A. E.
dc.contributor.authorArruda, P.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:50:09Z
dc.date.available2014-05-20T13:50:09Z
dc.date.issued2003-06-01
dc.description.abstractAn Arabidopsis thaliana cDNA clone encoding a plant uncoupling mitochondrial protein (AtPUMP1) was overexpressed in transgenic tobacco plants. Analysis of the AtPUMP1 mRNA content in the transgenic lines, determined by Northern blot, revealed variable levels of transgene expression. Antibody probing of Western blots of mitochondrial proteins from three independent transgenic lines showed significant accumulation of AtPUMP1 in this organelle. Overproduction of AtPUMP1 in transgenic tobacco plants led to a significant increase in tolerance to oxidative stress promoted by exogenous hydrogen peroxide as compared to wild-type control plants. These results provide the first biological evidence for a role of PUMP in protection of plant cells against oxidative stress damage.en
dc.description.affiliationUniv Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Genet, IB, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Patol Clin NMCE, FCM, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Genet & Evolucao, IB, Campinas, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Genet, IB, Botucatu, SP, Brazil
dc.format.extent203-209
dc.identifierhttp://dx.doi.org/10.1023/A:1024603530043
dc.identifier.citationJournal of Bioenergetics and Biomembranes. New York: Kluwer Academic/plenum Publ, v. 35, n. 3, p. 203-209, 2003.
dc.identifier.doi10.1023/A:1024603530043
dc.identifier.issn0145-479X
dc.identifier.lattes8649222099176162
dc.identifier.urihttp://hdl.handle.net/11449/17903
dc.identifier.wosWOS:000183994500002
dc.language.isoeng
dc.publisherKluwer Academic/plenum Publ
dc.relation.ispartofJournal of Bioenergetics and Biomembranes
dc.relation.ispartofjcr2.914
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjecttransgenic tobaccopt
dc.subjectplant uncoupling proteinpt
dc.subjectoxidative stresspt
dc.subjectconstitutive overexpressionpt
dc.titleOverexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stressen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
dcterms.rightsHolderKluwer Academic/plenum Publ
dspace.entity.typePublication
unesp.author.lattes8649222099176162
unesp.author.orcid0000-0002-3875-8664[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

Arquivos

Licença do Pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Botucatu - IBB - Instituto de Biociências