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Microbiological assay for the determination of azithromycin in ophthalmic solutions

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dc.contributor.authorSalgado, Hérida Regina Nunes [UNESP]
dc.contributor.authorRoncari, Ana Flávia Ferreira [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:21Z
dc.date.available2014-05-27T11:21:21Z
dc.date.issued2005-06-01
dc.description.abstractThe validation of a simple, sensitive and specific agar diffusion bioassay, applying cylinder-plate method, for the determination of the antibiotic azithromycin in ophthalmic solutions is described. Using a strain of Bacillus subtilis ATCC 9372 as the test organism, azithromycin at concentrations ranging from 50.0 to 200.0 μg·mL-1 could be measured in 1.666 7 mg·mL-1 ophthalmic solutions. A prospective validation of the method showed that the method was linear (r = 0.999 9) and precise (RSD = 0.70) and accurate (it measured the added quantities). The results obtained by bioassay method could be statistically calculated by linear parallel model and by means of regression analysis and verified using analysis of variance (ANOVA). We conclude that the microbiological assay is satisfactory for quantification of azithromycin in ophthalmic solutions.en
dc.description.affiliationPrograma de Pós-Graduação em Ciências Farmacêuticas Departamento de Fármacos e Medicamentos UNESP, Rodovia Araraquara-Jaú, km 1, Araraquara- SP-CEP 14801-902
dc.description.affiliationUnespPrograma de Pós-Graduação em Ciências Farmacêuticas Departamento de Fármacos e Medicamentos UNESP, Rodovia Araraquara-Jaú, km 1, Araraquara- SP-CEP 14801-902
dc.format.extent544-549
dc.identifier.citationYaoxue Xuebao, v. 40, n. 6, p. 544-549, 2005.
dc.identifier.issn0513-4870
dc.identifier.scopus2-s2.0-23044516230
dc.identifier.urihttp://hdl.handle.net/11449/68276
dc.language.isoeng
dc.relation.ispartofYaoxue Xuebao
dc.relation.ispartofsjr0,182
dc.rights.accessRightsAcesso restritopt
dc.sourceScopus
dc.subjectAzithromycin
dc.subjectCylinder-plate method
dc.subjectMicrobiological assay
dc.subjectOphthalmic solution
dc.subjectPharmaceutical dosage form
dc.subjectQuality control
dc.subjectantiinfective agent
dc.subjectazithromycin
dc.subjecteye drops
dc.subjectBacillus subtilis
dc.subjectchemistry
dc.subjectdrug effect
dc.subjectmicrobiological examination
dc.subjectquality control
dc.subjectAnti-Bacterial Agents
dc.subjectMicrobial Sensitivity Tests
dc.subjectOphthalmic Solutions
dc.subjectQuality Control
dc.titleMicrobiological assay for the determination of azithromycin in ophthalmic solutionsen
dc.typeArtigopt
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dspace.entity.typePublication
relation.isDepartmentOfPublicatione214da1b-9929-4ae9-b8fd-655e9bfeda4b
relation.isDepartmentOfPublication.latestForDiscoverye214da1b-9929-4ae9-b8fd-655e9bfeda4b
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentFármacos e Medicamentos - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas