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Progesterone upregulates GATA-1 on erythroid progenitors cells in liquid culture

dc.contributor.authorDuarte, ADS
dc.contributor.authorSales, TSI
dc.contributor.authorMengel, J. O.
dc.contributor.authorCosta, F. F.
dc.contributor.authorSaad, STO
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:43Z
dc.date.available2014-05-20T15:30:43Z
dc.date.issued2002-09-01
dc.description.abstractSteroids hormones modify the hematological features of homozygous sickle cell disease, including the levels of fetal hemoglobin. We used semi-quantitative RT-PCR analysis of GATA-1, GATA-2, NF-E2, and gamma-globin mRNA levels in a two-phase liquid culture system of human adult erythroid cells in order to assay the effect of progesterone upon gene expression. The levels of expression of GATA-1 and gamma-globin mRNA were significantly increased in cells treated with progesterone compared to untreated cells (1.7- to 2.0-fold). Progesterone treatment did not produce any stimulatory effect upon GATA-2 and NF-E2 mRNA expression. Differences in the synthesis of HbF protein could not be detected by flow cytometry, although we observed a small difference in mean intensity fluorescence between cells treated and cells untreated with progesterone on days 7 and 9. Using anti-transferrin receptor and anti-glycophorin A antibodies, we verified that addition of progesterone did not cause any change in erythroid proliferation and differentiation. In conclusion, it is possible that the increased expression of gamma-globin mRNA after progesterone treatment observed in this study may be related to the increased GATA-1 mRNA expression. Interactions of the steroid receptors with the basal transcriptional machinery and with transcription factors might mediate their transcriptional effects. (C) 2002 Elsevier B.V. (USA).en
dc.description.affiliationState Univ Campinas, Hematol & Hemotherapy Ctr, Campinas, SP, Brazil
dc.description.affiliationState Univ São Paulo, Dept Immunol, São Paulo, Brazil
dc.description.affiliationUnespState Univ São Paulo, Dept Immunol, São Paulo, Brazil
dc.format.extent213-224
dc.identifierhttp://dx.doi.org/10.1006/bcmd.2002.0551
dc.identifier.citationBlood Cells Molecules and Diseases. San Diego: Academic Press Inc. Elsevier B.V., v. 29, n. 2, p. 213-224, 2002.
dc.identifier.doi10.1006/bcmd.2002.0551
dc.identifier.issn1079-9796
dc.identifier.urihttp://hdl.handle.net/11449/40040
dc.identifier.wosWOS:000179991100009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBlood Cells Molecules and Diseases
dc.relation.ispartofjcr1.836
dc.relation.ispartofsjr0,828
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectHbFpt
dc.subjectprogesteronept
dc.subjecttranscription factorspt
dc.subjectRT-PCRpt
dc.subjectgene expressionpt
dc.titleProgesterone upregulates GATA-1 on erythroid progenitors cells in liquid cultureen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication

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