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Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology

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dc.contributor.authorMendes, Natalia J. [UNESP]
dc.contributor.authorCruz, Vanessa P. [UNESP]
dc.contributor.authorAshikaga, Fernando Y. [UNESP]
dc.contributor.authorCamargo, Samia M. [UNESP]
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.authorPiercy, Andrew N.
dc.contributor.authorBurgess, George H.
dc.contributor.authorCoelho, Rui
dc.contributor.authorSantos, Miguel N.
dc.contributor.authorMendonca, Fernando F.
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionValencia Coll
dc.contributor.institutionUniv Florida
dc.contributor.institutionPortuguese Inst Ocean & Atmosphere
dc.date.accessioned2018-11-26T17:06:08Z
dc.date.available2018-11-26T17:06:08Z
dc.date.issued2016-08-30
dc.description.abstractThe tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.en
dc.description.affiliationUniv Estadual Paulista, Lab Biol & Genet Peixes, Botucatu, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Marine Sci, Lab Genet Pesqueira & Conservacao, Santos, SP, Brazil
dc.description.affiliationValencia Coll, Lake Nona Campus, Orlando, FL USA
dc.description.affiliationUniv Florida, Florida Museum Nat Hist, Florida Program Shark Res, Gainesville, FL 32611 USA
dc.description.affiliationPortuguese Inst Ocean & Atmosphere, IPMA, Algarve, Portugal
dc.description.affiliationUnespUniv Estadual Paulista, Lab Biol & Genet Peixes, Botucatu, SP, Brazil
dc.description.sponsorshipInvestigador-FCT contract from the Portuguese Foundation for Science and Technology (FCT, Fundacao para a Ciencia e Tecnologia) - EU European Social Fund
dc.description.sponsorshipPrograma Operacional Potencial Humano
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdPrograma Operacional Potencial Humano: IF/00253/2014
dc.description.sponsorshipIdFAPESP: 2013/14555-4
dc.description.sponsorshipIdFAPESP: BIOTA 2011/23787-0
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.7717/peerj.2205
dc.identifier.citationPeerj. London: Peerj Inc, v. 4, 8 p., 2016.
dc.identifier.doi10.7717/peerj.2205
dc.identifier.fileWOS000383262700001.pdf
dc.identifier.issn2167-8359
dc.identifier.urihttp://hdl.handle.net/11449/161908
dc.identifier.wosWOS:000383262700001
dc.language.isoeng
dc.publisherPeerj Inc
dc.relation.ispartofPeerj
dc.relation.ispartofsjr1,087
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectHigh-throughput sequencing
dc.subjectGaleocerdo cuvier
dc.subjectShark
dc.subjectMicrosatellites
dc.subjectPopulation structure
dc.titleMicrosatellite loci in the tiger shark and cross-species amplification using pyrosequencing technologyen
dc.typeArtigo
dcterms.rightsHolderPeerj Inc
dspace.entity.typePublication
unesp.author.orcid0000-0003-3813-5157[8]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

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