Publicação:
Identification of sexually dimorphic gene expression in brain tissue of the fish Leporinus macrocephalus through mRNA differential display and real time PCR analyses

dc.contributor.authorAlves-Costa, Fernanda A. [UNESP]
dc.contributor.authorWasko, Adriane Pinto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:50:25Z
dc.date.available2014-05-20T13:50:25Z
dc.date.issued2010-03-01
dc.description.abstractDifferentially expressed genes in males and females of vertebrate species generally have been investigated in gonads and, to a lesser extent, in other tissues. Therefore, we attempted to identify sexually dimorphic gene expression in the brains of adult males and females of Leporinus macrocephalus, a gonochoristic fish species that presents a ZZ/ZW sex determination system, throughout a comparative analysis using differential display reverse transcriptase-PCR and real-time PCR. Four cDNA fragments were characterized, representing candidate genes with differential expression between the samples. Two of these fragments presented no significant identity with previously reported gene sequences. The other two fragments, isolated from male specimens, were associated to the gene that codes for the protein APBA2 (amyloid beta (A4) precursor protein-binding, family A, member 2) and to the Rab 37 gene, a member of the Ras oncogene family. The overexpression of these genes has been associated to a greater production of the beta-amyloid protein which, in turns, is the major factor that leads to Alzheimer's disease, and to the development of brain-tumors, respectively. Quantitative RT-PCR analyses revealed a higher Apba2 gene expression in males, thus validating the previous data on differential display. L. macrocephalus may represent an interesting animal model to the understanding of the function of several vertebrate genes, including those involved in neurodegenerative and cancer diseases.en
dc.description.affiliationUniv Estadual Paulista, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Genet, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent321-331
dc.identifierhttp://dx.doi.org/10.1007/s10709-009-9421-8
dc.identifier.citationGenetica. Dordrecht: Springer, v. 138, n. 3, p. 321-331, 2010.
dc.identifier.doi10.1007/s10709-009-9421-8
dc.identifier.issn0016-6707
dc.identifier.lattes1926863917378909
dc.identifier.orcid0000-0003-4600-0367
dc.identifier.urihttp://hdl.handle.net/11449/18000
dc.identifier.wosWOS:000274087700005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofGenetica
dc.relation.ispartofjcr1.366
dc.relation.ispartofsjr0,649
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectLeporinus macrocephalusen
dc.subjectDifferential displayen
dc.subjectReal time PCRen
dc.subjectApba2en
dc.subjectRab 37en
dc.subjectbeta-Amyloiden
dc.titleIdentification of sexually dimorphic gene expression in brain tissue of the fish Leporinus macrocephalus through mRNA differential display and real time PCR analysesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
dspace.entity.typePublication
unesp.author.lattes1926863917378909[2]
unesp.author.orcid0000-0003-4600-0367[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

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