Regulation of xylanase in Aspergillus phoenicis: a physiological and molecular approach
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Springer Heidelberg
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Microbial xylanolytic enzymes have a promising biotechnological potential, and are extensively applied in industries. In this study, induction of xylanolytic activity was examined in Aspergillus phoenicis. Xylanase activity induced by xylan, xylose or beta-methylxyloside was predominantly extracellular (93-97%). Addition of 1% glucose to media supplemented with xylan or xylose repressed xylanase production. Glucose repression was alleviated by addition of cAMP or dibutyryl-cAMP. These physiological observations were supported by a Northern analysis using part of the xylanase gene ApXLN as a probe. Gene transcription was shown to be induced by xylan, xylose, and beta-methylxyloside, and was repressed by the addition of 1% glucose. Glucose repression was partially relieved by addition of cAMP or dibutyryl cAMP.
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fungi, Aspergillus phoenicis, xylanase, cAMP, gene transcription
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Inglês
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Journal of Industrial Microbiology & Biotechnology. Heidelberg: Springer Heidelberg, v. 35, n. 4, p. 237-244, 2008.
