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Effect of cell cycle synchronization on the accuracy of murine and bovine embryo sex determination

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dc.contributor.authorLima, V. F. M. Hossepian de
dc.contributor.authorBem, A. R. de
dc.contributor.authorJorge, W. [UNESP]
dc.contributor.authorMoreira-Filho, C. A.
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:17:56Z
dc.date.available2014-05-27T11:17:56Z
dc.date.issued1994-02-01
dc.description.abstractDifferent cell cycle synchronization methods were used to increase the mitotic index and accuracy of sex determination in murine and bovine embryos. For sexing purposes, colchicine treatment for 2, 4, 6 and 8 h and the FdU-thymidine-colchicine combination were tested in murine embryos. The best results were obtained with colchicine treatment for 8 h (96.88% accuracy) and with FdU-thymidine-colchicine (97.22% accuracy). Mitotic indexes differed significantly between the 2 treatments (21.71% for colchicine and 32.95% for FdU-thymidine-colchicine). For sex identification of murine and bovine demi-embryos, both treatments were demonstrated to be equally effective (nearly 90%). The mitotic index for the FdU-treated murine demi-embryos (19.04%) was higher than the one obtained for the 8-h colchicine treatment (15.62%).en
dc.description.affiliationEMBRAPA-CENARGEN, Caixa Postal 02372, CEP 70770 Brasília, DF
dc.description.affiliationDepartamento de Genética, UNESP Botucatu, SP
dc.description.affiliationDepartamento de Imunologia, ICB-USP São Paulo, SP
dc.description.affiliationUnespDepartamento de Genética, UNESP Botucatu, SP
dc.format.extent521-534
dc.identifierhttp://dx.doi.org/10.1016/0093-691X(94)90088-Z
dc.identifier.citationTheriogenology. Woburn: Butterworth-heinemann, v. 41, n. 2, p. 521-534, 1994.
dc.identifier.doi10.1016/0093-691X(94)90088-Z
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-0027952151
dc.identifier.urihttp://hdl.handle.net/11449/130578
dc.identifier.wosWOS:A1994MY28900019
dc.language.isoeng
dc.publisherButterworth-heinemann
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectColchicine
dc.subjectEmbryo
dc.subjectFluordeoxyuridine
dc.subjectSexing
dc.subjectBovinae
dc.subjectMurinae
dc.titleEffect of cell cycle synchronization on the accuracy of murine and bovine embryo sex determinationen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderButterworth-heinemann
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

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