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Effect of cell cycle synchronization on the accuracy of murine and bovine embryo sex determination

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dc.contributor.authorLima, V. F. M. Hossepian de
dc.contributor.authorBem, A. R. de
dc.contributor.authorJorge, W. [UNESP]
dc.contributor.authorMoreira-Filho, C. A.
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:17:56Z
dc.date.available2014-05-27T11:17:56Z
dc.date.issued1994-02-01
dc.description.abstractDifferent cell cycle synchronization methods were used to increase the mitotic index and accuracy of sex determination in murine and bovine embryos. For sexing purposes, colchicine treatment for 2, 4, 6 and 8 h and the FdU-thymidine-colchicine combination were tested in murine embryos. The best results were obtained with colchicine treatment for 8 h (96.88% accuracy) and with FdU-thymidine-colchicine (97.22% accuracy). Mitotic indexes differed significantly between the 2 treatments (21.71% for colchicine and 32.95% for FdU-thymidine-colchicine). For sex identification of murine and bovine demi-embryos, both treatments were demonstrated to be equally effective (nearly 90%). The mitotic index for the FdU-treated murine demi-embryos (19.04%) was higher than the one obtained for the 8-h colchicine treatment (15.62%).en
dc.description.affiliationEMBRAPA-CENARGEN, Caixa Postal 02372, CEP 70770 Brasília, DF
dc.description.affiliationDepartamento de Genética, UNESP Botucatu, SP
dc.description.affiliationDepartamento de Imunologia, ICB-USP São Paulo, SP
dc.description.affiliationUnespDepartamento de Genética, UNESP Botucatu, SP
dc.format.extent521-534
dc.identifierhttp://dx.doi.org/10.1016/0093-691X(94)90088-Z
dc.identifier.citationTheriogenology. Woburn: Butterworth-heinemann, v. 41, n. 2, p. 521-534, 1994.
dc.identifier.dimensionspub.1046226437
dc.identifier.doi10.1016/0093-691X(94)90088-Z
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.orcid0000-0003-3433-4714
dc.identifier.pmid16727410
dc.identifier.scopus2-s2.0-0027952151
dc.identifier.urihttp://hdl.handle.net/11449/130578
dc.identifier.wosWOS:A1994MY28900019
dc.language.isoeng
dc.publisherButterworth-heinemann
dc.publisherElsevier
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restritopt
dc.sourceScopus
dc.sourceDimensions
dc.subjectColchicine
dc.subjectEmbryo
dc.subjectFluordeoxyuridine
dc.subjectSexing
dc.subjectBovinae
dc.subjectMurinae
dc.titleEffect of cell cycle synchronization on the accuracy of murine and bovine embryo sex determinationen
dc.typeArtigopt
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderButterworth-heinemann
dspace.entity.typePublication
relation.isOrgUnitOfPublicationab63624f-c491-4ac7-bd2c-767f17ac838d
relation.isOrgUnitOfPublication.latestForDiscoveryab63624f-c491-4ac7-bd2c-767f17ac838d
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
unesp.departmentGenética - IBBpt

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