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Isolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strain

dc.contributor.authorGonçalves, Fabiano Avelino
dc.contributor.authorLeite, Rodrigo Simões Ribeiro
dc.contributor.authorRodrigues, André [UNESP]
dc.contributor.authorArgandoña, Eliana Janet Sanjinez
dc.contributor.authorFonseca, Gustavo Graciano
dc.contributor.institutionFederal University of Grande Dourados
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T07:12:07Z
dc.date.available2022-04-29T07:12:07Z
dc.date.issued2013-10-01
dc.description.abstractThe aim of this work was to prospect for microorganisms in the local environment, selecting those with higher production of cellulolytic and hemicellulolytic enzymes. A total of 36 microorganisms were isolated in a sugarcane-based medium. Colonies were replated in medium containing carboxymethylcellulose as the main carbon source, where all grew, confirming their potential for plant biomass degradation. The enzyme production was evaluated by cultivating the isolated microorganisms in solid-state bioprocess using two different carbon sources, sugarcane bagasse and wheat bran. Among the microorganisms obtained, isolate 11, identified as Lichtheimia ramosa, presented a high level β-glucosidase production of 15.58. U/mL, as well as 2.41. U/mL of xylanase and 2.13. U/mL of CMCase when cultivated on wheat bran. Changes in temperature and cultivation time favored a higher production of β-glucosidase: 17.26. U/mL in wheat bran-based medium at 35. °C for 120. h. © 2013 Elsevier Ltd.en
dc.description.affiliationLaboratory of Bioengineering Faculty of Engineering Federal University of Grande Dourados, Dourados, Mato Grosso do Sul
dc.description.affiliationLaboratory of Enzymology Faculty of Biological and Environmental Sciences Federal University of Grande Dourados, Dourados, Mato Grosso do Sul
dc.description.affiliationLaboratory of Fungal Ecology and Systematics Department of Biochemistry and Microbiology São Paulo State University, Rio Claro, São Paulo
dc.description.affiliationLaboratory of Food Technology Faculty of Engineering Federal University of Grande Dourados, Dourados, Mato Grosso do Sul
dc.description.affiliationUnespLaboratory of Fungal Ecology and Systematics Department of Biochemistry and Microbiology São Paulo State University, Rio Claro, São Paulo
dc.format.extent377-384
dc.identifierhttp://dx.doi.org/10.1016/j.bcab.2013.06.006
dc.identifier.citationBiocatalysis and Agricultural Biotechnology, v. 2, n. 4, p. 377-384, 2013.
dc.identifier.doi10.1016/j.bcab.2013.06.006
dc.identifier.issn1878-8181
dc.identifier.scopus2-s2.0-84884245249
dc.identifier.urihttp://hdl.handle.net/11449/227207
dc.language.isoeng
dc.relation.ispartofBiocatalysis and Agricultural Biotechnology
dc.sourceScopus
dc.subjectScreening
dc.subjectSugarcane bagasse
dc.subjectWheat bran
dc.titleIsolation, identification and characterization of a novel high level β-glucosidase-producing Lichtheimia ramosa strainen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.departmentBioquímica e Microbiologia - IBpt

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