Publicação: Differential characterization of holocentric chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization
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2007-11-19
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A comparative study of holocentric chromosomes in the triatomine species Panstrongylus megistus, Rhodnius pallescens and Triatoma infestans was carried out in order to characterize heterochromatin, rDNA active sites and nucleolar proteins. Cytological preparations of seminiferous tubules were stained by silver impregnation, C banding, fluorochromes CMA 3/DA and DAPI/DA, and fluorescent in situ hybridization (FISH) with Drosophila melanogaster 28S rDNA probe. Our results showed interesting aspects of the organization of chromatin and chromosomes in the meiotic cells of these insects. In R. pallescens, sex chromosomes (X, Y) were distinct from autosomes, when submitted to silver impregnation, C banding, CMA 3 staining, and FISH, confirming that these chromosomes bear nucleolar organizer regions (NORs). In P. megistus, two of the three sex chromosomes were CMA 3/DAPI-; at early meiotic prophase and at diakinesis, silver impregnation corresponded with FISH signals, indicating that in this species, two chromosomes (probably a sex chromosome and an autosome) bear NORs. In T. infestans, silver nitrate and FISH also stained corresponding areas on meiotic chromosomes. Our data suggest that in triatomines, in general, the number and location of NORs are species-specific. These regions may be considered important chromosome markers for comparative studies to improve the understanding of evolutionary mechanisms in these hematophagous insects. ©FUNPEC-RP.
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CMA 3, DAPI, Fluorescent in situ hybridization, Holocentric chromosomes, Nucleolus organizer region, Triatomines, 4',6 diamidino 2 phenylindole, chromomycin A3, ribosome DNA, ribosome DNA 28s, silver, unclassified drug, autosome, chromosome, chromosome analysis, chromosome marker, chromosome NOR, DNA probe, Drosophila melanogaster, fluorescence in situ hybridization, Heteroptera, holocentric chromosome, meiosis, nonhuman, Panstrongylus, panstrongylus megistus, prophase, Rhodnius pallescense, sex chromosome, signal detection, staining, Triatoma infestans, Triatominae, Animals, Chromosomes, In Situ Hybridization, Fluorescence, Male, Rhodnius, Seminiferous Tubules, Staining and Labeling, Triatoma, Hexapoda, Panstrongylus megistus, Rhodnius pallescens
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Inglês
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Genetics and Molecular Research, v. 6, n. 3, p. 713-720, 2007.
