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Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus

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dc.contributor.authorGibertoni, A. M.
dc.contributor.authorMontassier, MDS
dc.contributor.authorSena, JAD
dc.contributor.authorGivisiez, PEN
dc.contributor.authorFuruyama, CRAG
dc.contributor.authorMontassier, H. J.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFundação Amparo Pesquisa Estado São Paulo
dc.date.accessioned2014-05-20T13:15:50Z
dc.date.available2014-05-20T13:15:50Z
dc.date.issued2005-04-01
dc.description.abstractA Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.en
dc.description.affiliationUniv Estadual Paulista, Lab Virol & Imunol, Dept Patol Vet, Fac Ciências Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Lab Genet Bacterias, Dept Biol Aplicada Agropecuaria, Fac Ciências Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationFundação Amparo Pesquisa Estado São Paulo, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Lab Virol & Imunol, Dept Patol Vet, Fac Ciências Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Lab Genet Bacterias, Dept Biol Aplicada Agropecuaria, Fac Ciências Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil
dc.format.extent1982-1984
dc.identifierhttp://dx.doi.org/10.1128/JCM.43.4.1982-1984.2005
dc.identifier.citationJournal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 4, p. 1982-1984, 2005.
dc.identifier.doi10.1128/JCM.43.4.1982-1984.2005
dc.identifier.fileWOS000228404100081.pdf
dc.identifier.issn0095-1137
dc.identifier.urihttp://hdl.handle.net/11449/2876
dc.identifier.wosWOS:000228404100081
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofJournal of Clinical Microbiology
dc.relation.ispartofjcr4.054
dc.relation.ispartofsjr2,256
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleDevelopment and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virusen
dc.typeArtigo
dcterms.licensehttp://journals.asm.org/site/misc/ASM_Author_Statement.xhtml
dcterms.rightsHolderAmer Soc Microbiology
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentBiologia - FCAVpt
unesp.departmentPatologia Veterinária - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias