Publicação: Detecção de Paracoccidioides sp. em amostras ambientais aerossóis
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Data
2012-02-24
Autores
Orientador
Bagagli, Eduardo 
Coorientador
Pós-graduação
Biologia Geral e Aplicada - IBB
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Tipo
Dissertação de mestrado
Direito de acesso
Acesso aberto
Resumo
Taking into account that paracoccidioidomycosis infection occurs by inhalation of the asexual conidia produced by Paracoccidioides sp. in its saprobic phase, this work presents the collection of aerosol samples as an option for environmental detection of this pathogen, by positioning a cyclonic air sampler at the entrance of armadillo burrows. Detection strategies included: direct culture, extinction technique culture and Nested PCR of the ITS1-5.8S-ITS2 region, as well as the evaluation of one armadillo (D. novemcinctus) as positive control for the studied area. Although the pathogen could not be isolated by the culturing strategies, the aerosol sampling associated with molecular detection by Nested PCR proved the best method for detecting Paracoccidioides sp. in the environment. Most of the ITS sequences obtained herein presented high similarity with the homologous sequences of P. lutzii from GenBank database, suggesting that this Paracoccidioides species may be not exclusive to mid-western Brazil as proposed so far
Descrição
Idioma
Português
Como citar
ARANTES, Thales Domingos. Detecção de Paracoccidioides sp. em amostras ambientais aerossóis. 2012. 65 f. Dissertação (mestrado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências de Botucatu, 2012.
