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Effects of oestrogen deficiency and 17 beta-estradiol therapy on bone healing in calvarial critical size defects treated with bovine bone graft

dc.contributor.authorAlmeida, Juliano Milanezi de [UNESP]
dc.contributor.authorBosco, Alvaro Francisco [UNESP]
dc.contributor.authorFaleiros, Paula Lazilha [UNESP]
dc.contributor.authorCabrera Pazmiño, Victor Fabrizio [UNESP]
dc.contributor.authorMurakawa, Ana Cristina [UNESP]
dc.contributor.authorBonfante, Samara [UNESP]
dc.contributor.authorNagata, Maria José Hitomi [UNESP]
dc.contributor.authorGarcia, Valdir Gouveia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCentro Universitário da Fundação Educacional de Barretos (UNIFEB)
dc.date.accessioned2015-10-21T13:10:13Z
dc.date.available2015-10-21T13:10:13Z
dc.date.issued2015-04-01
dc.description.abstractObjective: To histomorphometrically analyze the effect of ovariectomy-induced oestrogen deficiency and 17 beta-estradiol therapy on bone healing of surgically created critical-size defects (CSDs) treated with bovine bone graft (BBG).Methods: Forty-eight female rats were randomly assigned to the following 3 experimental groups (n = 16): sham-operated animals (SHAM), ovariectomized animals (OVX) and ovariectomized animals treated with oestrogen (OVX+E2). OVX+E2 animals received daily subcutaneous injections of 17 beta-estradiol (20 mu g/kg) from 8 days after ovariectomy until euthanasia. Thirty days after the surgery, an 8 mm CSD was surgically created in each calvaria of all animals and filled with BBG. Animals were euthanized at either 30 or 60 days postoperative. A histological analysis, percentage of Newly Formed Bone Area (NFBA), osteoblast and osteoclast number was histomorphometrically performed (p <= 0.05).Results: At 30 days, SHAM group (8.82% +/- 2.93) had significantly greater NFBA than OVX (4.66% +/- 1.35) and OVX+E2 groups (5.85% +/- 4.08) (p <= 0.05). At 60 days, SHAM group (11.51% +/- 3.08) and OVX+E2 group (9.84% +/- 1.87) had significantly greater NFBA than OVX animals (5.12% +/- 0.68) (p <= 0.05). Fewer osteoblasts were observed in the OVX group at 30 (763.40 +/- 121.60) and 60 (696.60 +/- 80.92) days than in the SHAM group at 30 days (1356.00 +/- 95.38). Fewer osteoclasts were observed in the OVX+E2 group (3.25 +/- 2.16) than in the SHAM (9.75 +/- 1.82) and OVX (12.75 +/- 1.47) groups at 30 days (p <= 0.05).Conclusions: Oestrogen deficiency compromises bone healing in calvarial CSDs treated with BBG in ovariectomized rats. After 60 days post-surgery, 17 beta-estradiol therapy improved bone healing in calvarial CSDs treated with BBG in ovariectomized rats.en
dc.description.affiliationCentro Universitário da Fundação Educacional de Barretos, Departamento de Periodontia, Faculdade de Odontologia de Barretos
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Cirurgia e Clínica Integrada, Faculdade de Odontologia de Araçatuba
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 08/04895-4
dc.format.extent631-641
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0003996915000205
dc.identifier.citationArchives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 60, n. 4, p. 631-641, 2015.
dc.identifier.doi10.1016/j.archoralbio.2015.01.009
dc.identifier.issn0003-9969
dc.identifier.lattes9831236034935598
dc.identifier.lattes8399870097572073
dc.identifier.lattes4068921369233125
dc.identifier.urihttp://hdl.handle.net/11449/128478
dc.identifier.wosWOS:000351790000015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofArchives Of Oral Biology
dc.relation.ispartofjcr2.050
dc.relation.ispartofsjr0,752
dc.rights.accessRightsAcesso restritopt
dc.sourceWeb of Science
dc.subjectBone graftingen
dc.subjectBone regenerationen
dc.subjectOestrogen replacement therapyen
dc.subjectCalvariaen
dc.titleEffects of oestrogen deficiency and 17 beta-estradiol therapy on bone healing in calvarial critical size defects treated with bovine bone graften
dc.typeArtigopt
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
relation.isOrgUnitOfPublication8b3335a4-1163-438a-a0e2-921a46e0380d
relation.isOrgUnitOfPublication.latestForDiscovery8b3335a4-1163-438a-a0e2-921a46e0380d
unesp.author.lattes9831236034935598
unesp.author.lattes8399870097572073
unesp.author.lattes4068921369233125
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araçatubapt
unesp.departmentCirurgia e Clínica Integrada - FOApt

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