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Trypanosoma cruzi: identification and characterization of a novel ribosomal protein L27 (TcrL27) that cross-reacts with an affinity-purified anti-Sm antibody

dc.contributor.authorPerone, D.
dc.contributor.authorSantos, MAM
dc.contributor.authorPeixoto, M. S.
dc.contributor.authorCicarelli, RMB
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:22:01Z
dc.date.available2014-05-20T15:22:01Z
dc.date.issued2003-06-01
dc.description.abstractSmall nuclear ribonucleoproteins (snRNPs)are involved in trans-splicing processing of pre-mRNA in Trypanosoma cruzi. To clone T. cruzi snRNPs we screened an epimastigote cDNA library with a purified antibody raised against the Sm-binding site of a yeast sequence. A clone was obtained containing a 507 bp-insert with an ORF of 399 bp and coding for a protein of 133 amino acids. Sequence analysis revealed high identity with the L27 ribosomal proteins from different species including: Canis familiaris, Homo sapiens, Schizosaccharomyces pombe and Saccharomyces cerevisiae. This protein has not been previously described in the literature and seems to be a new ribosomal protein in T. cruzi and was given the code TcrL27. To express this recombinant T. cruzi L27 ribosomal protein in E. coli, the insert was subcloned into the pET32a vector and a 26 kDa recombinant protein was purified. Immunoblotting studies demonstrated that this purified recombinant protein was recognized by the same anti-Sm serum used in the library screening as well as by chagasic and systemic lupus erythemathosus (SLE) sera. Our results suggest that the T. cruzi L27 ribosomal protein may be involved in autoimmunity of Chagas disease.en
dc.description.affiliationUNESP, Fac Ciências Farmaceut, Lab Immunol & Mol Biol Parasites, Dept Sci Biol, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Farmaceut, Lab Immunol & Mol Biol Parasites, Dept Sci Biol, BR-14801902 São Paulo, Brazil
dc.format.extent577-583
dc.identifierhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=152118&fulltextType=RA&fileId=S0031182003003159
dc.identifier.citationParasitology. New York: Cambridge Univ Press, v. 126, p. 577-583, 2003.
dc.identifier.doi10.1017/S0031182003003159
dc.identifier.fileWOS000187029300009.pdf
dc.identifier.issn0031-1820
dc.identifier.urihttp://hdl.handle.net/11449/33085
dc.identifier.wosWOS:000187029300009
dc.language.isoeng
dc.publisherCambridge University Press
dc.relation.ispartofParasitology
dc.relation.ispartofjcr2.511
dc.relation.ispartofsjr1,194
dc.rights.accessRightsAcesso abertopt
dc.sourceWeb of Science
dc.subjectTrypanosoma cruzipt
dc.subjectrecombinant proteinpt
dc.subjectimmunoscreeningpt
dc.subjectWestern blotpt
dc.subjectSouthern blotpt
dc.subjectTcrL27pt
dc.titleTrypanosoma cruzi: identification and characterization of a novel ribosomal protein L27 (TcrL27) that cross-reacts with an affinity-purified anti-Sm antibodyen
dc.typeArtigopt
dcterms.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dcterms.rightsHolderCambridge Univ Press
dspace.entity.typePublication
relation.isDepartmentOfPublication5004bcab-94af-4939-b980-091ae9d0a19e
relation.isDepartmentOfPublication.latestForDiscovery5004bcab-94af-4939-b980-091ae9d0a19e
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentCiências Biológicas - FCFpt

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