Publicação: eNOS T-786C polymorphism affects atorvastatin-induced changes in erythrocyte membrane fluidity
dc.contributor.author | Nagassaki, S. | |
dc.contributor.author | Herculano, R. D. | |
dc.contributor.author | Graeff, Carlos Frederico de Oliveira [UNESP] | |
dc.contributor.author | Tanus-Santos, J. E. | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T13:26:16Z | |
dc.date.available | 2014-05-20T13:26:16Z | |
dc.date.issued | 2009-04-01 | |
dc.description.abstract | Statins have pleiotropic effects, including endothelial nitric oxide synthase (eNOS) upregulation and increased nitric oxide formation, which can be modulated by a genetic polymorphism in the promoter region of the eNOS gene (T-786C). Here, we report our investigation of whether this polymorphism modulates the effects of atorvastatin on the fluidity of erythrocyte membranes.We genotyped 200 healthy subjects (males, 18-60 years of age) and then randomly selected 15 of these with the TT genotype and 15 with the CC genotype to receive placebo or atorvastatin (10 mg/day oral administration) for 14 days. Cell membrane fluidity was evaluated by electron paramagnetic resonance (EPR) and spin-labeling method. The EPR spectra were registered on a VARIAN-E4 spectrometer. Thiobarbituric acid-reactive species (TBA-RS) and plasma membrane cholesterol were determined in the erythrocytes.Atorvastatin reduced membrane fluidity in CC subjects (P < 0.05) but not in those with the TT genotype (P > 0.05). While no significant differences were found in plasma membrane cholesterol concentrations, higher TBA-RS concentrations were found in the CC subjects than in the TT subjects (P < 0.05).These findings suggest that a short treatment with atorvastatin is disadvantageous to subjects with the CC genotype for the T-786C polymorphism compared to those with TT genotype, at least in terms of the hemorheological properties of erythrocytes. | en |
dc.description.affiliation | Univ São Paulo, Fac Med Ribeirao Preto, Dept Farmacol, BR-14049900 Ribeirao Preto, SP, Brazil | |
dc.description.affiliation | Univ São Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Fis & Matemat, BR-14040901 Ribeirao Preto, SP, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, Fac Ciencias Bauru, Dept Fis, BR-17033360 Bauru, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Fac Ciencias Bauru, Dept Fis, BR-17033360 Bauru, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.format.extent | 385-392 | |
dc.identifier | http://dx.doi.org/10.1007/s00228-008-0602-7 | |
dc.identifier.citation | European Journal of Clinical Pharmacology. New York: Springer, v. 65, n. 4, p. 385-392, 2009. | |
dc.identifier.doi | 10.1007/s00228-008-0602-7 | |
dc.identifier.issn | 0031-6970 | |
dc.identifier.orcid | 0000-0003-0162-8273 | |
dc.identifier.uri | http://hdl.handle.net/11449/8438 | |
dc.identifier.wos | WOS:000264176100007 | |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.relation.ispartof | European Journal of Clinical Pharmacology | |
dc.relation.ispartofjcr | 2.679 | |
dc.relation.ispartofsjr | 1,159 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Endothelial nitric oxide synthase | en |
dc.subject | EPR | en |
dc.subject | Genotypes | en |
dc.subject | Nitric oxide | en |
dc.subject | Polymorphisms | en |
dc.subject | Statins | en |
dc.title | eNOS T-786C polymorphism affects atorvastatin-induced changes in erythrocyte membrane fluidity | en |
dc.type | Artigo | |
dcterms.license | http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 | |
dcterms.rightsHolder | Springer | |
dspace.entity.type | Publication | |
unesp.author.lattes | 5268607684223281[3] | |
unesp.author.orcid | 0000-0003-0162-8273[3] | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Ciências, Bauru | pt |
unesp.department | Física - FC | pt |
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