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Publicação:
Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish

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dc.contributor.authorGravato, Carlos
dc.contributor.authorAbe, Flávia Renata
dc.contributor.authorde Oliveira, Danielle Palma [UNESP]
dc.contributor.authorSoares, Amadeu M. V. M.
dc.contributor.authorDomingues, Inês
dc.contributor.institutionUniversidade de Lisboa
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade de Aveiro
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:20:50Z
dc.date.available2021-06-25T10:20:50Z
dc.date.issued2021-01-01
dc.description.abstractAcetylcholinesterase (AChE) is a useful biomarker for organophosphate and carbamate pesticides exposure. The inhibition of this enzyme has been associated with neurotoxicity and alterations at higher levels of biological organization, such as behavior and development impairments. In this chapter, we describe the methodologies for analyses of AChE activity in pools of 96 h of embryos of zebrafish (Danio rerio) using a spectrophotometric method adapted to 96-well microtiter plates.en
dc.description.affiliationFaculdade de Ciências & CESAM Universidade de Lisboa
dc.description.affiliationSchool of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo
dc.description.affiliationDepartment of Biology & CESAM Universidade de Aveiro Campus de Santiago
dc.description.affiliationNational Institute for Alternative Technologies ofremoval of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry
dc.description.affiliationUnespNational Institute for Alternative Technologies ofremoval of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of Chemistry
dc.format.extent119-124
dc.identifierhttp://dx.doi.org/10.1007/978-1-0716-1091-6_10
dc.identifier.citationMethods in Molecular Biology, v. 2240, p. 119-124.
dc.identifier.doi10.1007/978-1-0716-1091-6_10
dc.identifier.issn1940-6029
dc.identifier.issn1064-3745
dc.identifier.scopus2-s2.0-85099678031
dc.identifier.urihttp://hdl.handle.net/11449/205765
dc.language.isoeng
dc.relation.ispartofMethods in Molecular Biology
dc.sourceScopus
dc.subjectAChE
dc.subjectBiomarkers
dc.subjectDanio rerio
dc.subjectEllman’s technique
dc.subjectEmbryos
dc.subjectNeurotoxicity
dc.titleAcetylcholinesterase (AChE) Activity in Embryos of Zebrafishen
dc.typeCapítulo de livropt
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt

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Araraquara - IQAR - Instituto de Química