Can aPDT Provide Decontamination and Remineralization of Caries-Affected Dentin?

Abstract

Significance The affected caries, like dentin, should be preserved. Therefore, alternative techniques for removing and decontaminating dentin, such as aPDT, should be explored. Approach Bovine dentin specimens (n=159) were obtained. MIC and MBC of curcumin (1.25-320 µM) were determined and caries lesions induced by Streptococcus mutans/Actinomyces naeslundii. A spherical bur (SB), dentin excavator (DE) and ultrasound (US) were used to remove the infected dentin. Disinfection was performed using chlorhexidine (0.2%) and aPDT (320 µM, 5 min incubation, LED, 460 nm, 15, 60, and 100 J/cm², 22 mW/cm2). Cross-sectional hardness, Raman, confocal images and bond strength were performed. Results CSH increased with depth (p<0.05) and was unaffected by aPDT (p>0.05). aPDT decreased the carbonate/phosphate ratio (p<0.05) and effectively inactivated bacteria on both the surface and within the dentin tubules, without altering bond strength. Conclusions aPDT may serve as a vital tool for decontaminating and remineralizing affected dentin without compromising bond strength.