Avaliação da mangiferina na periodontite induzida em ratos
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Data
2008-03-28
Autores
Carvalho, Roney Rick de [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Na população mundial, existe uma elevada prevalência de periodontite que é responsável por grande parte da perda dentária nos indivíduos. Portanto, existe uma constante busca por novas opções terapêuticas mais eficazes e seguras para o tratamento de periodontite. Neste trabalho, avaliamos os efeitos de uma substância natural, a mangiferina - uma xantona glicosilada obtida da Mangifera indica, sobre a doença periodontal experimental induzida em ratos. Os mecanismos de ação pela qual a mangiferina exerce sua ação, também foram alvos deste trabalho, bem como os prováveis efeitos tóxicos decorrentes de seu tratamento (1, 4 ou 7 dias). A periodontite foi induzida em ratos machos Wistar (~180 g) aplicando-se uma ligadura ao redor do primeiro molar inferior direito. Os animais receberam diariamente pela via oral o veículo (salina 10 mL/kg), piroxicam (20 mg/kg) ou mangiferina (100 mg/kg). Nos dias 1, 4 ou 7 após a aplicação da ligadura, os animais foram mortos e a perda óssea alveolar (POA) foi determinada. Com a finalidade de elucidar os mecanismos de ação da mangiferina sobre a POA, realizamos as quantificações da celularidade presente nos tecidos gengivais, avaliamos a expressão de COX-2 neste tecido e determinamos o rolamento e adesão leucocitária. Também avaliamos a atuação da mangiferina sobre os níveis de lipoxina A4 e sobre a ação das metaloproteinases (MMPs) 2 e 9. A ativação da angiogênese e da proliferação celular também foi alvo de avaliação nos animais submetidos aos diferentes tratamentos. Os possíveis efeitos tóxicos da administração da mangiferina foram avaliados através da evolução do peso corporal, pesos dos órgãos vitais (coração, fígado, pulmões, rins e baço) e também pela determinação bioquímica (uréia, creatinina, gama-GT, ALT, AST e glicose) do soro dos animais. A administração oral de mangiferina reduziu a...
In the global population, there is an elevated prevalence of periodontitis that is responsible for a large portion of tooth loss in individuals, thus provoking a constant search for new therapeutic options that treat this affliction more efficaciously and safely. In the present work, we evaluate the effects of a natural substance, mangiferin - a glycosylated xanthone obtained from Mangifera indica - on periodontal disease experimentally induced in rats. The action mechanisms by which mangiferin exerts its action were also targets of our study, as well as the toxic effects resulting from resulting its application (1, 4 or 7 days). Periodontitis was induced in male Wistar rats (~180 g) by applying a ligature around the right inferior primary molar. The animals received the vehicle daily by the oral route (saline 10 mL/kg), piroxicam (20 mg/kg) or mangiferin (100 mg/kg). On days 1, 4 or 7 after ligature application, the animals were sacrificed and alveolar bone loss (ABL) was determined. In order to elucidate the mechanisms of mangiferin action on ABL, we quantified the cellularity present in gingival tissues, evaluated COX-2 expression in this tissue and determined rolling and leukocyte adhesion. We also evaluated the performance of mangiferin on lipoxin A4 levels and the action of metalloproteinases (MMPs) 2 and 9. Activation of angiogenesis and cellular proliferation were also targets of assessment in animals submitted to the different treatments. The possible toxic effects of mangiferin administration were evaluated through the evolution of body weight, vital organ weights (heart, liver, lungs, kidneys and spleen) and also by biochemical determination (urea, creatinine, gamma-GT, ALT, AST and glucose) in animal serum. Oral mangiferin administration reduced ABL after 1 day (p<0.05), 4 days (p<0.001) and 7 days (p<0.001) from periodontitis induction, compared to saline-treated animals... (Complete abstract click electronic access below)
In the global population, there is an elevated prevalence of periodontitis that is responsible for a large portion of tooth loss in individuals, thus provoking a constant search for new therapeutic options that treat this affliction more efficaciously and safely. In the present work, we evaluate the effects of a natural substance, mangiferin - a glycosylated xanthone obtained from Mangifera indica - on periodontal disease experimentally induced in rats. The action mechanisms by which mangiferin exerts its action were also targets of our study, as well as the toxic effects resulting from resulting its application (1, 4 or 7 days). Periodontitis was induced in male Wistar rats (~180 g) by applying a ligature around the right inferior primary molar. The animals received the vehicle daily by the oral route (saline 10 mL/kg), piroxicam (20 mg/kg) or mangiferin (100 mg/kg). On days 1, 4 or 7 after ligature application, the animals were sacrificed and alveolar bone loss (ABL) was determined. In order to elucidate the mechanisms of mangiferin action on ABL, we quantified the cellularity present in gingival tissues, evaluated COX-2 expression in this tissue and determined rolling and leukocyte adhesion. We also evaluated the performance of mangiferin on lipoxin A4 levels and the action of metalloproteinases (MMPs) 2 and 9. Activation of angiogenesis and cellular proliferation were also targets of assessment in animals submitted to the different treatments. The possible toxic effects of mangiferin administration were evaluated through the evolution of body weight, vital organ weights (heart, liver, lungs, kidneys and spleen) and also by biochemical determination (urea, creatinine, gamma-GT, ALT, AST and glucose) in animal serum. Oral mangiferin administration reduced ABL after 1 day (p<0.05), 4 days (p<0.001) and 7 days (p<0.001) from periodontitis induction, compared to saline-treated animals... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Periodontite - Aspectos farmacológicos - Estudos experimentais, Farmacologia
Como citar
CARVALHO, Roney Rick de. Avaliação da mangiferina na periodontite induzida em ratos. 2008. 84 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2008.