Análisis morfométrico, merístico, filogenético y filogeográfico del caballo de mar Hippocampus reidi (Ginsburg 1933) (Teleostei: Sygnathidae) de la costa nororiental da Venezuela
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Data
2010-11-08
Autores
Ron Esteves, Ernesto José [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Fue realizado el análisis morfométrico, merístico, morfológico y filogenético del caballo de mar Hippocampus reidi (Ginsburg 1933) (Teleostei: Syngnathidae) de la costa nororiental de Venezuela, mediante la aplicación de técnicas de taxonomía convencional y molecular utilizando fragmentos secuenciados de los genes Citocromo Oxidasa I y Citocromo B como marcadores genéticos. Los resultados indican que no es posible identificar las diferentes entidades taxonómicas de la región caribeña venezolana con base solamente en un solo tipo de caracteres, por lo que es necesario utilizar información merística, morfológica y morfométrica de forma conjunta para la identificación de las mismas, lográndose identificar las variables longitud del rostro (SnL), ancho del cuerpo en la región torácica (TW9), longitud de la cabeza (HL), altura de la coroneta (CH), número de espinas supra oculares (ES) y posición de los anillos prominentes en vista dorsal, como caracteres diagnósticos a nivel específico. La aplicación de las técnicas moleculares fue de suma utilidad en la correcta identificación y separación de los individuos de las dos especies, resaltando la importancia e utilidad de los genes Citocromo Oxidasa I y Citocromo B como marcadores moleculares para estas especies. Adicionalmente, fue realizado un análisis genético poblacional bajo el enfoque filogeográfico, con la especie Hippocampus reidi que resultó ser la más abundante en las regiones de muestreo, con la finalidad de comparar la diversidad genética, con base en la información obtenida de secuencias del gen Citocromo B del ADN mitocondrial, para determinar la estructura geográfica y proponer una hipótesis filogeográfica que establezca las relaciones entre y dentro de las poblaciones del Mar Caribe (Laguna de la Restinga, Laguna de las Marites, Golfo de Cariaco y Golfo de Venezuela) y la localidad de Natal... (Resumen completo clicar acceso eletrônico abajo)
Morphometric, meristic, morphological and phylogenetic analysis from the seahorse Hippocampus reidi (Ginsburg 1933) (Teleostei: Syngnathidae) from the northeastern coast of Venezuela was performed, through the application of techniques using conventional and molecular taxonomy from the sequenced fragments of genes Cytochrome Oxidase I and Cytochrome B as genetic markers. The results indicate that it is not possible to identify the different taxa of Venezuelan Caribbean region, based only on a single type of characters, so it is necessary to use information meristic, morphological and morphometric together to identify them, achieving identify the variables snout length (SnL), body width in the thoracic region (TW9), head length (HL), height of the coronet (CH), number of supra oculars spines (ES) and prominent position of the rings in dorsal view, as diagnostic features at specific level. The application of molecular techniques was very useful in the correct identification and separation of individuals of both species, highlighting the importance and usefulness of the genes Cytochrome Oxidase I and Cytochrome B as molecular markers. Additionally, we carried out a population genetic analysis under the phylogeographic approach, with the species Hippocampus reidi, that was the most abundant in the sampling regions, in order to compare the genetic diversity based on information obtained from the Cytochrome B gene mitochondrial DNA sequences to determine the geographical structure and propose a phylogeographic hypotheses in order to establish the relationship between and within populations of the Caribbean Sea (Laguna de la Restinga, Laguna de las Marites, Cariaco Gulf and Gulf of Venezuela) and the locality of Natal, Brazil, located in the Western Atlantic Ocean. The results of nucleotide diversity and haplotype diversity, together with the presence of several unique haplotypes in each... (Complete abstract click electronic access below)
Morphometric, meristic, morphological and phylogenetic analysis from the seahorse Hippocampus reidi (Ginsburg 1933) (Teleostei: Syngnathidae) from the northeastern coast of Venezuela was performed, through the application of techniques using conventional and molecular taxonomy from the sequenced fragments of genes Cytochrome Oxidase I and Cytochrome B as genetic markers. The results indicate that it is not possible to identify the different taxa of Venezuelan Caribbean region, based only on a single type of characters, so it is necessary to use information meristic, morphological and morphometric together to identify them, achieving identify the variables snout length (SnL), body width in the thoracic region (TW9), head length (HL), height of the coronet (CH), number of supra oculars spines (ES) and prominent position of the rings in dorsal view, as diagnostic features at specific level. The application of molecular techniques was very useful in the correct identification and separation of individuals of both species, highlighting the importance and usefulness of the genes Cytochrome Oxidase I and Cytochrome B as molecular markers. Additionally, we carried out a population genetic analysis under the phylogeographic approach, with the species Hippocampus reidi, that was the most abundant in the sampling regions, in order to compare the genetic diversity based on information obtained from the Cytochrome B gene mitochondrial DNA sequences to determine the geographical structure and propose a phylogeographic hypotheses in order to establish the relationship between and within populations of the Caribbean Sea (Laguna de la Restinga, Laguna de las Marites, Cariaco Gulf and Gulf of Venezuela) and the locality of Natal, Brazil, located in the Western Atlantic Ocean. The results of nucleotide diversity and haplotype diversity, together with the presence of several unique haplotypes in each... (Complete abstract click electronic access below)
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Palavras-chave
Peixe marinho - Filogeografia, Peixe - Genetica, Ecologia molecular, Molecular ecology
Como citar
RON ESTEVES, Ernesto José. Análisis morfométrico, merístico, filogenético y filogeográfico del caballo de mar Hippocampus reidi (Ginsburg 1933) (Teleostei: Sygnathidae) de la costa nororiental da Venezuela. 2010. 142 f. Tese (doutorado) - Universidade Estadual Paulista, Instituto de Biociências de Botucatu, 2010.