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dc.contributor.authorCastella, Gemma
dc.contributor.authorCoutinho, Selene Dall' Acqua [UNESP]
dc.contributor.authorJavier Cabanes, F.
dc.identifier.citationMedical Mycology. London: Informa Healthcare, v. 52, n. 1, p. 99-105, 2014.
dc.description.abstractMembers of the genus Malassezia are lipophilic basidiomycetous yeasts, which are part of the normal cutaneous microbiota of humans and other warm-blooded animals. Currently, this genus consists of 14 species that have been characterized by phenetic and molecular methods. Although several molecular methods have been used to identify and/or differentiate Malassezia species, the sequencing of the rRNA genes and the chitin synthase-2 gene (CHS2) are the most widely employed. There is little information about the beta-tubulin gene in the genus Malassezia, a gene has been used for the analysis of complex species groups. The aim of the present study was to sequence a fragment of the beta-tubulin gene of Malassezia species and analyze their phylogenetic relationship using a multilocus sequence approach based on two rRNA genes (ITS including 5.8S rRNA and D1/D2 region of 26S rRNA) together with two protein encoding genes (CHS2 and beta-tubulin). The phylogenetic study of the partial beta-tubulin gene sequences indicated that this molecular marker can be used to assess diversity and identify new species. The multilocus sequence analysis of the four loci provides robust support to delineate species at the terminal nodes and could help to estimate divergence times for the origin and diversification of Malassezia species.en
dc.description.sponsorshipPREI from the Universitat Autonoma de Barcelona
dc.publisherInforma Healthcare
dc.relation.ispartofMedical Mycology
dc.sourceWeb of Science
dc.subjectbeta tubulin geneen
dc.subjectphylogenetic studyen
dc.subjectmultilocus sequence analysisen
dc.titlePhylogenetic relationships of Malassezia species based on multilocus sequence analysisen
dcterms.rightsHolderInforma Healthcare
dc.contributor.institutionUniv Autonoma Barcelona
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniv Autonoma Barcelona, Dept Anim Hlth & Anat, Vet Mycol Grp, E-08193 Barcelona, Spain
dc.description.affiliationUniv Estadual Paulista, Lab Mol & Cellular Biol, Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Lab Mol & Cellular Biol, Sao Paulo, Brazil
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