Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture
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Data
2014-01-01
Autores
Mauro, M. O.
Pesarini, J. R.
Marin-Morales, M. A. [UNESP]
Monreal, M. T. F. D.
Monreal, A. C. D.
Mantovani, M. S.
Oliveira, R. J.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Funpec-editora
Resumo
This study evaluated the mutagenicity and antimutagenicity of inulin in a chromosomal aberration assay in cultures of the meristematic cells of Allium cepa. The treatments evaluated were as follows: negative control - seed germination in distilled water; positive control - aqueous solution of methyl methanesulfonate (10 mu g/mL MMS); mutagenicity - aqueous solutions of inulin (0.015, 0.15, and 1.50 mu g/mL); and antimutagenicity - associations between MMS and the different inulin concentrations. The antimutagenicity protocols established were pretreatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The damage reduction percentage (DR%) was 43.56, 27.77, and 55.92% for the pre-treatment; -31.11, 18.51, and 7.03% for the simultaneous simple; 30.43, 19.12, and 21.11% for the simultaneous with pre-incubation; and 64.07, 42.96, and 53.70% for the post-treatment. The results indicated that the most effective treatment for inhibiting damages caused by MMS was the post-treatment, which was followed by the pretreatment, suggesting activity by bioantimutagenesis and desmutagenesis. The Allium cepa assay was demonstrated to be a good screening test for this type of activity because it is easy to perform, has a low cost, and shows DR% that is comparable to that reported studies that evaluated the prevention of DNA damage in mammals by inulin.
Descrição
Palavras-chave
Inulin, Fiber, Antimutagenic activity
Como citar
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 3, p. 4808-4819, 2014.