Publicação: Diferenciação osteogênica de células mesenquimais do cordão umbilical canino
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Data
Autores
Orientador
Andrade, Alexandre Lima de 
Coorientador
Pós-graduação
Ciência Animal - FMVA
Curso de graduação
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ISSN da Revista
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Editor
Universidade Estadual Paulista (Unesp)
Tipo
Tese de doutorado
Direito de acesso
Acesso aberto
Resumo
This study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBM
Descrição
Palavras-chave
Cão, Fator de crescimento transformador beta, Ossos - Crescimento, Plasma Rico em Plaquetas, Células mesenquimais estromais, Geleia de Wharton, Cordão umbilical, Canine umbilical cord
Idioma
Português
Como citar
SOUZA, Talita Floering Brêda. Diferenciação osteogênica de células mesenquimais do cordão umbilical canino. 2013. 72 f. , 2013.
