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dc.contributor.authorFerrarezzi, Ana Lucia
dc.contributor.authorPiveta, Daniele H.
dc.contributor.authorBonilla-Rodriguez, Gustavo Orlando [UNESP]
dc.contributor.authorSilva, Roberto da [UNESP]
dc.contributor.authorGuisan, Jose Manuel
dc.contributor.authorGomes, Eleni [UNESP]
dc.contributor.authorPessela, Benevides Costa
dc.date.accessioned2015-04-27T11:55:57Z
dc.date.available2015-04-27T11:55:57Z
dc.date.issued2013
dc.identifier.citationAdvances in Enzyme Research, v. 01, n.4, p. 79-90, 2013.
dc.identifier.issn2328-4846
dc.identifier.urihttp://hdl.handle.net/11449/122670
dc.description.abstractLipases have important applications in biotechnological processes, motivating us to produce, purify, immobilize and perform a biochemical characterization of the lipase from Rhizomucor pusillus. The fungus was cultivated by solid state fermentation producing lipolytic activity of about 0.5 U/mL(4U/g). A partial purification by gel filtration chromatography in Se-phacryl S-100 allowed obtaining a yield of about 85% and a purification factor of 5.7. Our results revealed that the purified enzyme is very stable with some significant differences in its properties when compared to crude extract. The crude enzyme extract has an optimum pH and temperature of 7.5 ° C and 40 ° C, respectively. After purification, a shift of the optimum pH from 7 to 8 was observed, as well as a rise in optimumtemperature to 60 ° C and an increase in stability. The enzyme was immobilized on CNBr-Agarose and Octyl-Agarose supports, having the highest immobilization yield of 94% in the second resin. The major advantage of immobilization in hydrophobic media such as Octyl is in its hyper activation, which in this case was over 200%, a very interesting finding. Another advantage of this type of immobilization is the possibility of using the derivatives in biotechnological applications, such as in oil enriched with omega-3 as the results obtained in this study display the hydrolysis of 40% EPA and 7% DHA from sardine oil, promising results compared to the literature.en
dc.format.extent79-90
dc.language.isoeng
dc.relation.ispartofAdvances in Enzyme Research
dc.sourceCurrículo Lattes
dc.subjectEnzyme Immobilizationen
dc.subjectSolid State Fermentationen
dc.subjectPurificationen
dc.subjectn-3 Polyunsatured Fatty Acidsen
dc.subjectRhizomucor pusillusen
dc.titlePartial purification, immobilization and preliminary biochemical characterization of lipases from Rhizomucor pusilluspt
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstitute of Catalysis and Petrochemistry, CSIC, Campus UAM, Cantoblanco, Madrid, Spain
dc.contributor.institutionResearch Institute for Food Science, CIAL-CSIC, CalleNicolás Cabrera 9, Campus UAM, Madrid, Spain
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, RUA CRISTOVAO COLOMBO, 2265, JARDIM NAZARETH, CEP 15054-000, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, Sao Jose do Rio Preto, RUA CRISTOVAO COLOMBO, 2265, JARDIM NAZARETH, CEP 15054-000, SP, Brasil
dc.description.affiliationUnespDepartamento de Química e Ciências Ambientais, IBILCE UNESP - São José do Rio Preto.
dc.identifier.doi10.4236/aer.2013.14009
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
dc.identifier.fileISSN2328-4846-2013-01-04-79-90.pdf
dc.identifier.lattes9424175688206545
dc.identifier.lattes6955258588672130
dc.identifier.lattes7091241742851920
unesp.departmentBiologiapt
unesp.author.lattes9424175688206545
unesp.author.lattes6955258588672130
unesp.author.lattes7091241742851920
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