Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssoptfolia L. on HepG2 cells
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Data
2015-07-21
Autores
Araujo, Silvany de Sousa
Casimiro Fernandes, Thais Cristina [UNESP]
Cardona, Yaliana Tafurt [UNESP]
Almeida, Pedro Marcos de
Mann-Morales, Maria Aparecida [UNESP]
Santos, Andrea Vidal dos
Randau, Karina Perrelli
Benko-Iseppon, Ana Maria
Brasileiro-Vidal, Ana Christina
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Título de Volume
Editor
Elsevier B.V.
Resumo
Ethnopharmacological relevance: Euphorbia hyssopifolia L. is a weed with recognized antimicrobial potential employed in Indian, Asian and Latin-American popular medicine. However, little is known with regard to its toxic potential. The present study aimed to investigate the cytotoxic and genotoxic effects of ethanolic extract of E. hyssopifolia in HepG2 cell culture.Materials and methods: Phytochemical screening of ethanolic extract was carried out to determine the presence of active secondary plant metabolites. Six concentrations (0.00001, 0.0001, 0.001, 0.01, 0.1 and 1.0 mg/mL) of ethanolic extract were tested by the MTT assay to verify cytotoxicity. Then, genotoxic evaluations (alkaline comet assay and cytokinesis-block micronucleus assay - CBMN) were carried out in HepG2 cells with extract concentrations of 0.01, 0.1 and 1.0 mg/mL.Results: Mono and sesquiterpenes, triterpenes and steroids, and flavonoids were the main classes found in the phytochemical screening. Extract concentrations used in the MTT assay showed no cytotoxic activity. On the other hand, genotoxic activity was verified at 0.1 and 1.0 mg/mL in the alkaline comet assay. Additionally, the 1.0 mg/mL concentration induced severe cell damage leading to death in the CBMN assay, indicating a cytotoxic effect for this concentration in the latter method.Conclusion: The use of E. hyssopifolia extract for medicinal purposes should be avoided, because concentrations above 0.01 mg/mL may pose risk to human health due to cytotoxic and/or genotoxic effects. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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Palavras-chave
Antimicrobial, Botany, Cytotoxicity, Mutagenesis, Traditional medicine Africa, Traditional medicine meso- and Southern, America, Ethanolic extract, Comet assay, Micronucleus, Cell death, Euphorbiaceae
Como citar
Journal Of Ethnopharmacology. Clare: Elsevier Ireland Ltd, v. 170, p. 16-19, 2015.