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dc.contributor.authorLindae, Antje [UNESP]
dc.contributor.authorEberle, Raphael J. [UNESP]
dc.contributor.authorCaruso, Icaro P. [UNESP]
dc.contributor.authorCoronado, Monika A. [UNESP]
dc.contributor.authorMoraes, Fabio R. de [UNESP]
dc.contributor.authorAzevedo, Vasco
dc.contributor.authorArni, Raghuvir K. [UNESP]
dc.identifier.citationProtein Expression And Purification. San Diego: Academic Press Inc Elsevier Science, v. 112, p. 15-20, 2015.
dc.description.abstractThe gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable economical loss. Cold shock proteins are conserved in many bacteria and eukaryotic cells and they help to restore normal cell functions after cold shock in which some appear to have specific functions at normal growth temperature as well.Cold shock protein A from C pseudotuberculosis was expressed in Escherichia coli and purified. The thermal unfolding/refolding process characterized by circular dichroism, differential scanning calorimetry and NMR spectroscopy techniques indicated that the refolding process was almost completely reversible. (C) 2015 Elsevier Inc. All rights reserved.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.publisherElsevier B.V.
dc.relation.ispartofProtein Expression And Purification
dc.sourceWeb of Science
dc.subjectC. pseudotuberculosisen
dc.subjectCold shock proteinen
dc.subjectSpectroscopic methodsen
dc.subjectSecondary structureen
dc.subjectFolding and refolding proteinsen
dc.titleExpression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosisen
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.description.affiliationUniv Estadual Paulista UNESP, Ctr Multiusuario Inovacao Biomol, Dept Fis, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Lab Genet Celular &Mol, Inst Ciencias Biol, BR-270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Ctr Multiusuario Inovacao Biomol, Departamento de Física, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt[5][6][2]
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