Influence of culture medium and age of bovine blastocysts in established colonies of embryonic stem cells
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Data
2015
Autores
Guastali, Midyan Daroz [UNESP]
Monteiro, Bianca Andriolo [UNESP]
Maziero, Rosiara Rasária D. [UNESP]
Paschoal, Daniela Martins [UNESP]
Sudano, Mateus José [UNESP]
Rascado, Tatiana da Silva [UNESP]
Lima Neto, João Ferreira [UNESP]
Vergara, Luis Eduardo [UNESP]
Crocomo, Letícia Ferrari [UNESP]
Maserati, Marc
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Resumo
The objective of this study was to evaluate the potential of bovine IVF blastocysts at different stages of embryonic development in establishing ESC-like. Furthermore, blastocysts cultured in medium containing (10%) and (2.5%) fetal calf serum (FCS) were compared to determine if the serum concentration during in vitro culture alters the blastocyst's potential to establish ESC-like culture. It was observed that only ICM's from expanded blastocysts adhered to the monolayer (n=160) independent of the concentration of serum used during IVF culture. There were no observable differences in potential to establish ESC-like in embryos cultured with 2,5% or 10% FCS . The bFGF didn´t seems to be required for maintenance of bovine ESC-like regardless of culture conditions. Blastocysts and colonies in primary culture and after the first passage were positive for Oct4, Nanog, SSEA-3 and TRA-1-81. Expanded blastocysts gave rise to ESC-like colonies, and the addition of LIF was sufficient to maintain cells undifferentiated in culture.
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Embryonic stem cells, Bovine, Blastocyst, Fetal calf serum, LIF
Como citar
Journal of Stem Cells, v. 9, n. 4, p. 225-234, 2015.