Estudo de tripanosomatídeos em búfalos domésticos (Bubalus bubalis)
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Data
2018-03-02
Autores
Romariz, Ana Paula Peres Lopes
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Tripanosomatídeos são protozoários parasitas flagelados que em ruminantes no Brasil incluem os pertencentes à família Trypanosomatidae, com os gêneros Trypanosoma e Leishmania. Com relação aos búfalos domésticos (Bubalus bubalis), pouco ou nada se conhece sobre estes parasitas. Este estudo teve como objetivo avaliar por meio de métodos parasitológicos (exame direto do parasita no microscópio), sorológicos (RIFI – reação de imunofluorescência indireta) e moleculares (PCR – reação em cadeia pela polimerase e sequenciamento) a presença de tripanosomatídeos em búfalos. Amostras de sangue e suabe conjuntival foram coletadas de 100 búfalos da Fazenda São Francisco, Andradina, SP e de 10 búfalos de Selvíria, MS. Dos 110 búfalos, 60,9% (67/110) apresentaram hemoflagelares tripanossomatídeos nos esfregaços sanguíneos e 90% (99/110) deles apresentaram anticorpos anti-Leishmania pela RIFI. O DNA destes parasitas foi detectado em 29,09% (32/110) dos búfalos pela técnica de PCR, sendo, 26,36% (29/110) na PCR do sangue e 3,6% (4/110) na PCR do suabe conjuntival. Das amostras positivas para PCR, sete foram enviadas para o sequenciamento gênico, e uma delas foi identificada como Leishmania infantum e três como Trypanosoma theileri, ambas as amostras apresentaram 100% de similaridade com as respectivas espécies após comparação pelo GenBank. Este estudo relata o primeiro registro de infecção por L. infantum em B. bubalis na região de Andradina, SP, e a presença de búfalos infectados por espécies de T. theileri, evidenciando a necessidade de mais estudos sobre tripanosomatídeos em búfalos.
Trypanosomatides are flagellate protozoan parasites that in ruminants include those belonging to the family Trypanosomatidae, with the genera Trypanosoma and Leishmania, but with little or nothing information about these parasite infections in domestic water buffalo (Bubalus bubalis). Tthis study was assessed through the parasitological (direct examination of the parasite under microscope), serological (IFAT – immunofluorescence antibody test), and molecular tests (PCR from blood and conjunctival swab and genic sequencing) to detect possible trypanosomatides in buffalo herds from Andradina, SP and Selvíria, MS. Thus, 60.9% (67/110) buffaloes presented trypanosomatid parasites in the blood smears, 90% (99/110) had anti-Leishmania antibodies by IFAT. The DNA of these parasites was detected in 29.09% (32/110) of buffaloes by the PCR technique; 26.36% (29/110) in blood and 3.6% (4/110) in conjunctival swab. Positive PCR samples from seven animals were sent for DNA sequencing, and one of them was identified as Leishmania infantum and three as Trypanosoma theileri, both samples showed 100% of similarity with the respective species after comparison by BenBank. This study reports for the first time the presence of parasite L. infantum in B. bubalis from Andradina, SP, and highlights the presence of buffaloes infected with T. theileri, and indicates the need for more studies on trypanosomatid parasites in buffaloes.
Trypanosomatides are flagellate protozoan parasites that in ruminants include those belonging to the family Trypanosomatidae, with the genera Trypanosoma and Leishmania, but with little or nothing information about these parasite infections in domestic water buffalo (Bubalus bubalis). Tthis study was assessed through the parasitological (direct examination of the parasite under microscope), serological (IFAT – immunofluorescence antibody test), and molecular tests (PCR from blood and conjunctival swab and genic sequencing) to detect possible trypanosomatides in buffalo herds from Andradina, SP and Selvíria, MS. Thus, 60.9% (67/110) buffaloes presented trypanosomatid parasites in the blood smears, 90% (99/110) had anti-Leishmania antibodies by IFAT. The DNA of these parasites was detected in 29.09% (32/110) of buffaloes by the PCR technique; 26.36% (29/110) in blood and 3.6% (4/110) in conjunctival swab. Positive PCR samples from seven animals were sent for DNA sequencing, and one of them was identified as Leishmania infantum and three as Trypanosoma theileri, both samples showed 100% of similarity with the respective species after comparison by BenBank. This study reports for the first time the presence of parasite L. infantum in B. bubalis from Andradina, SP, and highlights the presence of buffaloes infected with T. theileri, and indicates the need for more studies on trypanosomatid parasites in buffaloes.
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Palavras-chave
Trypanosoma Theiler, Leishmania infantum, RIFI, Búfalos, Parasitologicos, PCR, Trypanosoma theileri, IFAT, Water buffalo, Parasitological