Effectiveness of photodynamic therapy associated with irrigants over two biofilm models
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Data
2017-12-01
Autores
Rosa, Ricardo Abreu da
Santini, Manuela Favarin
Poli de Figueiredo, Jose Antonio
Visioli, Fernando.
Pereira, Jefferson Ricardo
Vivan, Rodrigo Ricci [UNESP]
Montagner, Francisco
Reis So, Marcus Vinicius
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Background: This study aimed to evaluate the antibacterial effect and the biofilm disruption promoted by antimicrobial photodynamic therapy (aPDT) associated with sodium hypochlorite (NaOCl) and chiorexidine (CHX) over monospecies and multispecies biofilms. Methods: In monospecies model, forty-six premolars were inoculated with Enterococcus faecalis for 21 days and divided into three groups: saline, CHX and NaOCl. After irrigation, aPDT was performed. Samples were collected at baseline (S1) and after irrigation (S2) and aPDT (S3). Colony-forming unit (CFU) counts were performed. In multispecies model, sixty bovine dentin blocks were infected intraorally for 72 h and divided into six groups: saline, saline/aPDT, CHX, CHX/aPDT, NaOCl and NaOCl/aPDT. The percentage and the biovolume of live cells and the total biovolume were assessed using confocal laser scanning microscopy. Results: CHX and NaOCl showed the lowest CFU counts (P < 0.05). aPDT reduced the bacterial counts in saline (S2-S3; P < 0.05). The lowest amount of live cells was observed in CHX, CHX/aPDT, NaOCl and NaOCl/aPDT. aPDT did not reduce the total biovolume (P > 0.05). Conclusion: aPDT associated with saline reduced the bacterial load in root canals infected with E. faecalis. aPDT did not reduce the total biovolume in situ; however, the irrigant was decisive to disrupt multispecies biofilms.
Descrição
Palavras-chave
Photodynamic therapy, Enterococcus faecalis, Root canal irrigants, Biofilm, Confocal laser scanning microscopy
Como citar
Photodiagnosis And Photodynamic Therapy. Amsterdam: Elsevier Science Bv, v. 20, p. 169-174, 2017.