Protective effects of spray-dried açaí (Euterpe oleracea Mart) fruit pulp against initiation step of colon carcinogenesis
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Data
2015-11-01
Autores
Romualdo, Guilherme Ribeiro [UNESP]
Fragoso, Mariana Franco [UNESP]
Borguini, Renata Galhardo
de Araújo Santiago, Manuela Cristina Pessanha
Fernandes, Ana Angélica Henrique [UNESP]
Barbisan, Luis Fernando [UNESP]
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Resumo
The present study aims to evaluate whether açaí (Euterpe oleracea Mart) fruit pulp powder (AP) feeding attenuates the initiation step of chemically-induced mouse colon carcinogenesis. Male Swiss mice were fed low fat diet containing 2.5% or 5.0% of AP (weeks 1 to 4) produced by a spray-drying system. All groups received an intraperitoneal (i.p.) injection of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at week 3. After the first administration of carcinogen, blood samples were collected to perform Single Cell Gel Electrophoresis Assay. Some mice were euthanized at week 3 (n = 5 mice/group) and liver samples were collected for immunohistochemical and glutathione analysis. Othermice received a second i.p. injection of AOM at week 4 and were fed a high-fat diet to accelerate the development of preneoplastic aberrant crypt foci (ACF) until week 14 (n = 10 mice/group). Cyanidin 3-glucoside and cyanidin 3-rutinoside, lutein, a-carotene and ß-carotene were identified as the main anthocyanins and carotenoids in AP, respectively. At week 3, both dietary AP interventions (2.5% or 5.0%) reduced (p < 0.001) peripheral blood cell DNA damage induced by AOM. Moreover, dietary 5.0% AP increased (p = 0.002) hepatic total glutathione. At week 14, 5.0% AP intake reduced (p < 0.05) ACFmultiplicity. The findings indicate that AP feeding attenuates chemically-induced mouse colon carcinogenesis by increasing total GSH and attenuating DNA damage and preneoplastic lesion development.
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Azoxymethane, Açaí (Euterpe oleracea Mart.), Colon carcinogenesis, DNA damage, Male Swiss mice
Como citar
Food Research International, v. 77, p. 432-440.