Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth
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Data
2016-06-01
Autores
Dias, Kássia de Carvalho [UNESP]
Barbugli, Paula Aboud [UNESP]
Vergani, Carlos Eduardo [UNESP]
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Resumo
This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.
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Palavras-chave
Candida albicans, Cell survival, HEPES, Keratinocytes, Staphylococcus aureus
Como citar
Journal of Microbiological Methods, v. 125, p. 40-42.