Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules
Carregando...
Arquivos
Data
2017-02-15
Autores
Ben Aissa, A.
Herrera-Chacon, A.
Pupin, R. R. [UNESP]
Sotomayor, M. D.P.T. [UNESP]
Pividori, M. I.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4 °C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets.
Descrição
Palavras-chave
biotinylated biomolecules, immunomagnetic separation, magnetic molecularly-imprinted polymer, magnetic particles, magneto-actuated immunoassay
Como citar
Biosensors and Bioelectronics, v. 88, p. 101-108.