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  • ItemArtigo
    The Transcriptomic Landscape of Age-Induced Changes in Human Visceral Fat and the Predicted Omentum-Liver Connectome in Males
    (2023-05-01) Moraes, Diogo de [UNESP]; Mousovich-Neto, Felippe; Cury, Sarah Santiloni [UNESP]; Oliveira, Jakeline [UNESP]; Souza, Jeferson dos Santos [UNESP]; Freire, Paula Paccielli [UNESP]; Dal-Pai-Silva, Maeli [UNESP]; Mori, Marcelo Alves da Silva; Fernandez, Geysson Javier; Carvalho, Robson Francisco [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Estadual de Campinas (UNICAMP); Universidad de Antioquia (UdeA)
    Aging causes alterations in body composition. Specifically, visceral fat mass increases with age and is associated with age-related diseases. The pathogenic potential of visceral fat accumulation has been associated with its anatomical location and metabolic activity. Visceral fat may control systemic metabolism by secreting molecules that act in distal tissues, mainly the liver, through the portal vein. Currently, little is known about age-related changes in visceral fat in humans. Aiming to identify molecular and cellular changes occurring with aging in the visceral fat of humans, we analyzed publicly available transcriptomic data of 355 omentum samples from the Genotype-Tissue Expression portal (GTEx) of 20–79-year-old males and females. We identified the functional enrichment of genes associated with aging, inferred age-related changes in visceral fat cellularity by deconvolution analysis, profiled the senescence-associated secretory phenotype of visceral adipose tissue, and predicted the connectivity of the age-induced visceral fat secretome with the liver. We demonstrate that age induces alterations in visceral fat cellularity, synchronous to changes in metabolic pathways and a shift toward a pro-inflammatory secretory phenotype. Furthermore, our approach identified candidates such as ADIPOQ-ADIPOR1/ADIPOR2, FCN2-LPR1, and TF-TFR2 to mediate visceral fat-liver crosstalk in the context of aging. These findings cast light on how alterations in visceral fat with aging contribute to liver dysfunction and age-related disease etiology.
  • ItemArtigo
    Characterization of repetitive DNA on the genome of the marsh rat Holochilus nanus (Cricetidae: Sigmodontinae)
    (2023-01-01) do Nascimento Moreira, Camila [UNESP]; Cardoso, Adauto Lima [UNESP]; Valeri, Mirela Pelizaro; Ventura, Karen; Ferguson-Smith, Malcolm Andrew; Yonenaga-Yassuda, Yatiyo; Svartman, Marta; Martins, Cesar [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de Minas Gerais (UFMG); Universidade de São Paulo (USP); University of Cambridge
    Repetitive DNA are sequences repeated hundreds or thousands of times and an abundant part of eukaryotic genomes. SatDNA represents the majority of the repetitive sequences, followed by transposable elements. The species Holochilus nanus (HNA) belongs to the rodent tribe Oryzomyini, the most taxonomically diverse of Sigmodontinae subfamily. Cytogenetic studies on Oryzomyini reflect such diversity by revealing an exceptional range of karyotype variability. However, little is known about the repetitive DNA content and its involvement in chromosomal diversification of these species. In the search for a more detailed understanding about the composition of repetitive DNA on the genome of HNA and other species of Oryzomyini, we employed a combination of bioinformatic, cytogenetic and molecular techniques to characterize the repetitive DNA content of these species. RepeatExplorer analysis showed that almost half of repetitive content of HNA genome are composed by Long Terminal Repeats and a less significant portion are composed by Short Interspersed Nuclear Elements and Long Interspersed Nuclear Elements. RepeatMasker showed that more than 30% of HNA genome are composed by repetitive sequences, with two main waves of repetitive element insertion. It was also possible to identify a satellite DNA sequence present in the centromeric region of Oryzomyini species, and a repetitive sequence enriched on the long arm of HNA X chromosome. Also, comparative analysis between HNA genome with and without B chromosome did not evidence any repeat element enriched on the supernumerary, suggesting that B chromosome of HNA is composed by a fraction of repeats from all the genome.
  • ItemArtigo
    Integrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiae
    (2023-03-01) Wolf, Ivan Rodrigo [UNESP]; Marques, Lucas Farinazzo [UNESP]; de Almeida, Lauana Fogaça [UNESP]; Lázari, Lucas Cardoso [UNESP]; de Moraes, Leonardo Nazário [UNESP]; Cardoso, Luiz Henrique [UNESP]; Alves, Camila Cristina de Oliveira [UNESP]; Nakajima, Rafael Takahiro [UNESP]; Schnepper, Amanda Piveta [UNESP]; Golim, Marjorie de Assis [UNESP]; Cataldi, Thais Regiani; Nijland, Jeroen G.; Pinto, Camila Moreira [UNESP]; Fioretto, Matheus Naia [UNESP]; Almeida, Rodrigo Oliveira; Driessen, Arnold J. M.; Simōes, Rafael Plana [UNESP]; Labate, Mônica Veneziano; Grotto, Rejane Maria Tommasini [UNESP]; Labate, Carlos Alberto; Fernandes Junior, Ary [UNESP]; Justulin, Luis Antonio [UNESP]; Coan, Rafael Luiz Buogo [UNESP]; Ramos, Érica [UNESP]; Furtado, Fabiana Barcelos [UNESP]; Martins, Cesar [UNESP]; Valente, Guilherme Targino; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); University of Groningen; Ciência e Tecnologia do Sudeste de Minas Gerais; Max Planck Institute for Heart and Lung Research
    Ethanol (EtOH) alters many cellular processes in yeast. An integrated view of different EtOH-tolerant phenotypes and their long noncoding RNAs (lncRNAs) is not yet available. Here, large-scale data integration showed the core EtOH-responsive pathways, lncRNAs, and triggers of higher (HT) and lower (LT) EtOH-tolerant phenotypes. LncRNAs act in a strain-specific manner in the EtOH stress response. Network and omics analyses revealed that cells prepare for stress relief by favoring activation of life-essential systems. Therefore, longevity, peroxisomal, energy, lipid, and RNA/protein metabolisms are the core processes that drive EtOH tolerance. By integrating omics, network analysis, and several other experiments, we showed how the HT and LT phenotypes may arise: (1) the divergence occurs after cell signaling reaches the longevity and peroxisomal pathways, with CTA1 and ROS playing key roles; (2) signals reaching essential ribosomal and RNA pathways via SUI2 enhance the divergence; (3) specific lipid metabolism pathways also act on phenotype-specific profiles; (4) HTs take greater advantage of degradation and membraneless structures to cope with EtOH stress; and (5) our EtOH stress-buffering model suggests that diauxic shift drives EtOH buffering through an energy burst, mainly in HTs. Finally, critical genes, pathways, and the first models including lncRNAs to describe nuances of EtOH tolerance are reported here.
  • ItemArtigo
    Gestational exposure to continuous light impairs the development of the female reproductive system in adult Wistar rat offspring
    (2023-04-15) Ogo, Fernanda Mithie; Siervo, Glaucia Eloisa Munhoz Lion; Praxedes, Ana Maria; Vieira, Henrique Rodrigues; da Silva Scarton, Suellen Ribeiro; Bitencourt, Ana Tereza Guimarães; Arena, Arielle Cristina [UNESP]; Simão, Andréa Name Colado; Guerra, Marina Trevizan; de Freitas Mathias, Paulo Cesar; Fernandes, Glaura Scantamburlo Alves; Universidade Estadual de Londrina (UEL); State University of Maringá; State University of Western Parana; Universidade Estadual Paulista (UNESP); Universidade Federal de Mato Grosso do Sul (UFMS)
    Introduction: It has been suggested that maternal exposure to constant light during the gestational period could be considered as a chronic stressor, impairing offspring development by interfering in neuroendocrine and behavior responses. Objective: This study aimed to evaluate whether maternal exposure to continuous light during pregnancy affects the adult reproductive system in the female offspring. Materials and Methods: Pregnant Wistar rats were allocated into light–dark (LD) group, exposed to light and dark photoperiod during gestation, and the light–light (LL) group, exposed to a photoperiod of constant light during gestation. After birth, pups were maintained under normal light–dark photoperiod until adulthood. At postnatal day 90, blood was collected from the female offspring, to analyze plasma luteinizing hormone (LH) and progesterone levels, and the uterus and ovaries were harvested for morphometric, histological, and oxidative stress evaluations. Results and Discussion: Female exposure to continuous light during the intrauterine period resulted in the adult reduction of LH and increased progesterone plasma levels, and uterine injuries a higher number of endometrial glands and reduced levels of antioxidant enzymes, such as glutathione reductase and glutathione S-transferase. In these experimental conditions, gestational continuous light exposure disturbs sex hormone balance and reduces the antioxidant enzymatic activity in the uterus of female offspring in adult life.
  • ItemArtigo
    Histological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolis
    (2022-11-01) Conceição, Mariana [UNESP]; Gushiken, Lucas Fernando Sérgio [UNESP]; Aldana-Mejía, Jennyfer Andrea; Tanimoto, Matheus Hikaru; Ferreira, Marcos Vital de Sá [UNESP]; Alves, Andreia Cristina Miranda [UNESP]; Miyashita, Marina Naomi [UNESP]; Bastos, Jairo Kenupp; Beserra, Fernando Pereira [UNESP]; Pellizzon, Cláudia Helena [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
    Skin wound healing is a complex process that requires the mutual work of cellular and molecular agents to promote tissue restoration. In order to improve such a process, especially in cases of impaired healing (e.g., diabetic ulcer, chronic wounds), there is a search for substances with healing properties and low toxicity: two features that some natural products—such as the bee product named propolis—exhibit. Propolis is a resinous substance obtained from plant resins and exudates with antioxidant, anti-inflammatory, and antitumoral activities, among other biological ones. Based on the previously reported healing actions of different types of propolis, the Brazilian red propolis (BRP) was tested for this matter. A skin wound excision model in male Wistar rats was performed using two topical formulations with 1% red propolis as treatments: hydroalcoholic extract and Paste. Macroscopical, histological and immunohistochemical analysis were performed, revealing that red propolis enhanced wound contraction, epithelialization, reduced crust formation, and modulated the distribution of healing associated factors, mainly collagen I, collagen III, MMP-9, TGF-β3 and VEGF. Biochemical analysis with the antioxidants SOD, MPO, GSH and GR showed that propolis acts similarly to the positive control, collagenase, increasing these molecules’ activity. These results suggest that BRP promotes enhanced wound healing by modulating growth factors and antioxidant molecules related to cutaneous wound healing.
  • ItemArtigo
    Is lactational sertraline exposure safe for maternal health and the reproductive/neurobehavioral development of the descendants? A study in rats
    (2023-04-01) Lozano, Ana Flávia Quiarato [UNESP]; Moura, Mayara Silva [UNESP]; Dinhani, Taís Raquel Batisoco [UNESP]; Valencise, Lethícia [UNESP]; De Grava Kempinas, Wilma [UNESP]; Universidade Estadual Paulista (UNESP)
    Although sertraline is considered one of the safest antidepressants in the lactation period, there are still few studies that assess its impact on child development. Therefore, this experimental study aimed to clarify the effect of sertraline on the neurobehavioral and reproductive development of male rats. Thus, 30 lactating rats were divided into 3 experimental groups (n = 10/group): CO- received filtered water, S10 and S20 groups that received, respectively, 10 and 20 mg/kg/day of sertraline. Treatment was performed by gavage, from postnatal days (PND) 1–20. During this period, the reflex and somatic development of rats were observed, as well as maternal behavior. On PND 21, mothers were euthanized and the organs were weighed. On PND 21, 45, and 100, one male from each litter was euthanized for histological and immunohistochemical (PCNA and WT1) analysis of the reproductive organs. The growth of body weight, the anogenital distance (AGD), the time to puberty, sperm quality, sexual behavior, neurobehavior, and natural fertility were also verified. Statistical analysis: One-way ANOVA or Kruskal-Wallis test (p ≤ 0.05). The results showed that mothers in the S20 group had an increase in thyroid weight. The male offspring exposed to sertraline had lower body weight (PND 7), lower AGD (PND 7 and 14), and delay in reflex development, in addition to histological alterations in the testis (PND 21). In adulthood, sperm quality was altered, without compromising natural fertility. Therefore, the present study found important alterations in the reflex and reproductive development of male rats exposed to sertraline during lactation.
  • ItemArtigo
    SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study
    (2023-04-01) Rosa, Josiane de Lima [UNESP]; Figueiredo, Thamiris Moreira [UNESP]; Barros, Jorge Willian Franco [UNESP]; dos Santos, Driéle Bretone [UNESP]; de Deus, Ramão Souza [UNESP]; Borges, Cibele Santos [UNESP]; Kempinas, Wilma De Grava [UNESP]; Klinefelter, Gary Robert; Universidade Estadual Paulista (UNESP); Research Triangle Park
    Infertility affects approximately 15% of couples of reproductive age, and 50% of the cases are directly related to men. The evaluation of male fertility is based on analyses of routine seminal parameters and the use of more advanced techniques can help identify fertility biomarkers. SP22 sperm protein is considered a biomarker in murine species since its concentration is highly correlated with sperm fertility. As the role of this protein as a biomarker is already well-established in other species, we hypothesized that this same correlation could apply to human. Thus, the present study aimed to investigate possible correlations between SP22 concentration and sperm parameters in fertile and infertile men. For this, a study was carried out on 21 volunteers’ seminal samples who were grouped according to fertility as fertile (n = 10) or infertile (n = 11). Conventional and functional sperm analyses, membrane protein extraction, quantification and immunolocalization of SP22 were performed. The infertile volunteers showed an increase in the percentage of sperm with abnormalities in head morphology and a decrease in the percentage of sperm with intact plasma membrane and damaged acrosomal membrane. Serum concentration of the hormone SHBG was also decreased in infertile volunteers. The damage to the plasma membrane was positively correlated with the superoxide anion production. Although none of the functional parameters were correlated with SP22 concentration, type D sperm motility was negatively correlated and type A+B sperm motility was positively correlated. This preliminary study opens new paths in the characterization of SP22 as a non-invasive biomarker for predicting fertility/infertility.
  • ItemArtigo
    Reproductive Development of Male Rats Exposed In Utero to Stress and/or Sertraline
    (2022-12-01) Quiarato Lozano, Ana Flávia [UNESP]; Marques Tavares, Bruna [UNESP]; Villela E Silva, Patrícia [UNESP]; Franco De Barros, Jorge Willian [UNESP]; De Grava Kempinas, Wilma [UNESP]; Universidade Estadual Paulista (UNESP)
    Despite increased prescription of sertraline during pregnancy, little is known about its action on reproductive development. Therefore, this study aimed to investigate the impact that stress, associated or not with sertraline, causes on the reproductive development of male rats. Pregnant Wistar rats were divided into 4 groups (n = 16/group): CO - received filtered water; SE - received 20 mg/kg sertraline; ST - submitted to restraint stress and received filtered water; SS - submitted to restraint stress and received sertraline. The treatment was carried out from gestational days (GDs) 13-20. The animals were euthanized on GD 20 (n = 8/group), postnatal day (PND) 45 (n = 8/group), and PND 110 (n = 8/group). The testes and epididymis were analyzed histologically, and immunohistochemistry was performed on the testes by proliferating cell nuclear antigen (PCNA) and the Wilms tumor protein (Wt1). Sperm quality was also analyzed on PND 110. The evolution of body weight, anogenital distance (AGD), and puberty installation day were also verified. Statistical analysis: 2-way ANOVA or Kruskal-Wallis test (p ≤. 05). Fetal testes presented a large number of acidophilic cells in the sertraline-exposed groups. The SS group also showed a decrease in the nuclear volume of Leydig cells. This same group showed low expression of PCNA and Wt1, decreased weight of the testes and epididymis, lower AGD, and delayed puberty installation. The adulthood groups exposed to sertraline presented alterations in sperm morphology and motility. The results demonstrated that prenatal exposure to sertraline compromises the development of the rat reproductive system.
  • ItemArtigo
    Prenatal exposure to sertraline, associated or not with stress, can negatively program somatic and neurobehavioral development of female rats, and dysregulate reproductive function in adulthood
    (2023-03-01) Moura, Mayara Silva [UNESP]; Lozano, Ana Flávia Quiarato [UNESP]; Tavares, Bruna Marques [UNESP]; Figueiredo, Thamíris Moreira [UNESP]; Franco de Barros, Jorge Willian [UNESP]; Valencise, Lethicia [UNESP]; de Grava Kempinas, Wilma [UNESP]; Universidade Estadual Paulista (UNESP)
    Selective serotonin reuptake inhibitors (SSRIs) are prescribed to pregnant women for treating mental illnesses. Among the drugs of this class, sertraline (ST) is the antidepressant therapy recommended most frequently. Therefore, this study aimed to evaluate the impact of gestational ST treatment on reproductive parameters and toxicological target organs of rat female offspring, as well as on somatic, reflex and neurobehavioral development, in a model of maternal adversity. Pregnant Wistar rats received vehicle (filtered water) or ST hydrochloride (20 mg/Kg/day diluted in vehicle) by oral gavage, associated or not with restraint stress for 1 h/day from gestational days 13–20. F1 female offspring was evaluated on reproductive parameters, body weight and somatic and reflex milestones from postnatal day (PND) 1. On PNDs 25 and 72, the elevated-plus-maze test was performed, while toxicological target organs were evaluated on PNDs 42 and 80. In utero exposure to ST, regardless of exposure to stress, reduced body weight at birth and affected the somatic development and estrous cycle. The absolute and relative thyroid weights were increased in Stress/ST group during puberty and adulthood, while the percentage of ovarian structures and the absolute uterine weight were altered in this group on PND 80. Prenatal exposure only to ST reduced initial body weight gain, delayed fur development and increased anxiety-like behavior on PND 25. Thus, this experimental study suggests that intrauterine exposure to ST disrupts the fetal environment and can negatively program serotonin-regulated processes. Furthermore, it impacts thyroid weight when associated with stress.
  • ItemEditorial
    Viral infections, vaccines and antiviral drugs in pregnancy and the development of the conceptus
    (2023-01-01) Paumgartten, Francisco J.R.; De Grava Kempinas, Wilma [UNESP]; Shiota, Kohei; Oswaldo Cruz Foundation; Universidade Estadual Paulista (UNESP); Kyoto University
  • ItemArtigo
    Reproductive outcomes of neonatal exposure to betamethasone in male and female rats
    (2023-05-01) Figueiredo, Thamiris Moreira [UNESP]; de Barros, Jorge Willian Franco [UNESP]; dos Santos Borges, Cibele [UNESP]; Pacheco, Tainá Louise [UNESP]; de Lima Rosa, Josiane [UNESP]; Anselmo-Franci, Janete Aparecida; Kempinas, Wilma De Grava [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
    Betamethasone (BM) is the drug of choice for antenatal corticosteroid therapy for women at risk of preterm delivery because it induces fetal lung maturation and enhances survival after birth. However, our group reported evidence of fetal programming and impaired reproductive development and function in rats exposed during the critical window of genital system development. Therefore, we aimed to investigate the effects of BM on the sexual development of rats in the period that corresponds to antenatal corticosteroid therapy in humans. Male and female rats were exposed subcutaneously to BM at 0.1 μg/g of pups' body weight or to a NaCl 0.9% solution (control) on postnatal days 1–3. It was observed that neonatal exposure to BM decreased body weight and weight gain in male and female rats during treatment. The estrous cycle was deregulated and LH level was decreased in female rats. In male rats, the sperm concentration in the caput–corpus of the epididymis was decreased, whereas the sperm transit time and sperm concentration in the cauda of the epididymis were increased. Our results demonstrated that neonatal exposure to BM impaired body growth of male and female rats, deregulated the estrous cycle of female rats, and altered sperm quality of male rats. Therefore, BM exposure from postnatal days 1 to 3 corroborated results previously observed after prenatal exposure to this drug. Despite the recognized importance of human antenatal corticosteroid therapy, the findings of this study should encourage further studies in order to minimize possible adverse postnatal effects.
  • ItemArtigo
    Antinociceptive and anti-inflammatory properties of aqueous extract obtained from Serjania marginata Casar leaves
    (2023-03-25) Matos Leitão, Maicon; Euclides Silva-Filho, Saulo; Arena, Arielle Cristina [UNESP]; Heredia-Vieira, Silvia Cristina; Cardoso, Cláudia Andrea Lima; Kassuya, Cândida Aparecida Leite; Faculty of Health Sciences; Unigran Capital University Center; Federal University of Mato Grosso Do Sul; Universidade Estadual Paulista (UNESP); Anhanguera-Uniderp University; State University of Mato Grosso do Sul
    Ethnopharmacological relevance: Serjania marginata Casar (Sapindaceae Family) Leaves are popularly used against abdominal pain. Antiulcer properties of S. marginata were scientifically described, however rare studies showed the antinociceptive effects of this plant. Aim of study: In this study, we investigated the antinociceptive and anti-inflammatory effects of aqueous extract obtained from Serjania marginata leaves (AESM) in nociception/inflammation models. Material and methods: AESM was analyzed in FIA-ESI-IT-MS and Mass spectrometer LTQ XL. AESM oral administration (p.o.) (30, 100 and 300 mg/kg), dexamethasone subcutaneous injection (1 mg/kg, s.c.) and morphine (5 mg/kg, s.c.) were tested against the acetic acid-induced nociception, carrageenan-induced acute inflammatory paw edema/hyperalgesia, formalin-induced nociception and carrageenan-induced pleurisy in Swiss mice. Results: Flavonoids rutin was detected in the phytochemical analysis of this extract. Oral treatment of AESM 300 mg/kg significantly reduced the number of acetic acid-induced abdominal writhing. AESM (100 and 300 mg/kg) significantly inhibited formalin-induced nociception, mechanical hyperalgesia and paw edema in carrageenan-model. Furthermore, AESM significantly inhibited leukocyte migration and protein exudation in the carrageenan-induced pleurisy test. Conclusion: This study confirms the antinociceptive, and anti-inflammatory activity of AESM, which may explain, in part, the popular use of this plant as a natural antinociceptive agent. This pharmacological action can be caused by flavonoids such as rutin and other compounds present in AESM.
  • ItemResenha
    Guttiferones: An insight into occurrence, biosynthesis, and their broad spectrum of pharmacological activities
    (2023-01-25) Conceição, Mariana [UNESP]; Beserra, Fernando Pereira [UNESP]; Aldana Mejia, Jennyfer Andrea; Caldas, Gabriel Rocha; Tanimoto, Matheus Hikaru; Luzenti, Andréia Marincek; Gaspari, Priscyla Daniely Marcato; Evans, Nicholas David; Bastos, Jairo Kenupp; Pellizzon, Cláudia Helena [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); Southampton General Hospital
    Guttiferones belong to the polyisoprenylated benzophenone, a class of compounds, a very restricted group of natural plant products, especially in the Clusiaceae family. They are commonly found in bark, stem, leaves, and fruits of plants of the genus Garcinia and Symphonia. Guttiferones have the following classifications according to their chemical structure: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, and T. All of them have received growing attention due to its multiple biological activities. This review provides a first comprehensive approach to plant sources, phytochemical profile, specific pharmacological effects, and mechanisms of guttiferones already described. Studies indicate a broad spectrum of pharmacological activities, such as: anti-inflammatory, immunomodulatory, antioxidant, antitumor, antiparasitic, antiviral, and antimicrobial. Despite the low toxicity of these compounds in healthy cells, there is a lack of studies in the literature related to toxicity in general. Given their beneficial effects, guttiferones are expected to be great potential drug candidates for treating cancer and infectious and transmissible diseases. However, further studies are needed to elucidate their toxicity, specific molecular mechanisms and targets, and to perform more in-depth pharmacokinetic studies. This review highlights chemical properties, biological characteristics, and mechanisms of action so far, offering a broad view of the subject and perspectives for the future of guttiferones in therapeutics.
  • ItemArtigo
    Identification of Novel Genes Associated with Fish Skeletal Muscle Adaptation during Fasting and Refeeding Based on a Meta-Analysis
    (2022-12-01) Perez, Érika Stefani [UNESP]; Cury, Sarah Santiloni [UNESP]; Zanella, Bruna Tereza Thomazini [UNESP]; Carvalho, Robson Francisco [UNESP]; Duran, Bruno Oliveira Silva; Dal-Pai-Silva, Maeli [UNESP]; Universidade Estadual Paulista (UNESP); Federal University of Goias (UFG)
    The regulation of the fish phenotype and muscle growth is influenced by fasting and refeeding periods, which occur in nature and are commonly applied in fish farming. However, the regulators associated with the muscle responses to these manipulations of food availability have not been fully characterized. We aimed to identify novel genes associated with fish skeletal muscle adaptation during fasting and refeeding based on a meta-analysis. Genes related to translational and proliferative machinery were investigated in pacus (Piaractus mesopotamicus) subjected to fasting (four and fifteen days) and refeeding (six hours, three and fifteen days). Our results showed that different fasting and refeeding periods modulate the expression of the genes mtor, rps27a, eef1a2, and cdkn1a. These alterations can indicate the possible protection of the muscle phenotype, in addition to adaptive responses that prioritize energy and substrate savings over cell division, a process regulated by ccnd1. Our study reveals the potential of meta-analysis for the identification of muscle growth regulators and provides new information on muscle responses to fasting and refeeding in fish that are of economic importance to aquaculture.
  • ItemArtigo
    Analgesic and anti-inflammatory potential of ethanolic extract from Serjania erecta leaves
    (2023-03-01) Bernal, Laura Priscila Toledo; Leitão, Maicon Matos; Radai, Joyce Alencar Santos; Cardoso, Claudia Andrea Lima [UNESP]; Lencina, Joyce dos Santos; Fraga, Thiago Leite; Arena, Arielle Cristina [UNESP]; Silva-Filho, Saulo Euclides; Kassuya, Cândida Aparecida Leite; Federal University of Grande Dourados; University Center Unigran (UNIGRAN); Universidade Estadual Paulista (UNESP); Universidade Federal de Mato Grosso do Sul (UFMS)
    Ethnopharmacological relevance: The infusion of Serjania erecta Radlk (Sapindaceae) (popular name “cipó-cinco-folhas”) leaves is used in popular medicine to treat back pain. The anti-inflammatory, anti-hyperalgesic and anti-nociceptive properties of the ethanolic extract from S. erecta leaves (EESE) has not been yet completely clarified. Aim of the study: The present study investigated the anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties of EESE in experimental models in mice. Material and methods: EESE was fractionated by chromatographic techniques and the compound was identified by nuclear magnetic resonance (NMR), infrared (IR) spectrum, ultraviolet (UV) methods. Mice received a single dose of EESE by oral route (30, 100, and 300 mg/kg, p.o.) and were submitted to nociception induced by formalin, pleurisy induced by carrageenan and peritonitis induced by zymosan models. Mice also received EESE (30 and 100 mg/kg, p.o.) for 22 days in Complete Freund Adjuvant (CFA) model and another group received EESE for 7 days (30 and 100 mg/kg, p.o.) in pleurisy induced by Bacillus Calmette-Guerin (BCG). The cytotoxicity (MTT), phagocytic and chemotactic inhibitory activities of EESE were performed in in vitro assays. Results: The fractionation of EESE led to the identification of kaempferol-3-O-α-L-rhamnopyranoside. The oral administration of all doses of EESE decreased the nociceptive response induced by formalin. EESE significantly inhibited leukocyte migration in carrageenan-induced pleurisy and zymosan peritonitis models. The daily administration of EESE during for 7 days inhibited the leukocyte migration and the mycobacteria growth of pleural material obtained from animals which received BCG. EESE significantly reduced edema, cold allodynia and mechanical hyperalgesia responses induced by CFA. EESE did not induce cytotoxicity, and also decreased the leukocyte phagocytic activity, as well as, neutrophil chemotaxis. Conclusions: EESE showed analgesic and anti-inflammatory properties in acute and persistent experimental models in mice. EESE also reduced in vitro leukocyte chemotaxis and phagocytic activity without inducing cytotoxicity. The continuous oral treatment with EESE was effective against hyperalgesia and inflammation and these results could explain the popular use of S. erecta as an analgesic natural agent.
  • ItemArtigo
    miR-18a-5p Is Involved in the Developmental Origin of Prostate Cancer in Maternally Malnourished Offspring Rats: A DOHaD Approach
    (2022-12-01) Santos, Sergio Alexandre Alcantara [UNESP]; Portela, Luiz Marcos Frediani [UNESP]; Camargo, Ana Carolina Lima [UNESP]; Constantino, Flavia Bessi [UNESP]; Colombelli, Ketlin Thassiani [UNESP]; Fioretto, Matheus Naia [UNESP]; Mattos, Renato [UNESP]; de Almeida Fantinatti, Bruno Evaristo [UNESP]; Denti, Michela Alessandra; Piazza, Silvano; Felisbino, Sérgio Luis [UNESP]; Zambrano, Elena; Justulin, Luis Antonio [UNESP]; Universidade Estadual Paulista (UNESP); Fox Chase Cancer Center; University of Trento; Instituto Nacional de Ciencias Médicas y Nutrición
    The Developmental Origins of Health and Disease (DOHaD) concept correlates early life exposure to stressor conditions with the increased incidence of non-communicable chronic diseases, including prostate cancer (PCa), throughout the life span. However, the molecular mechanisms involved in this process remain poorly understood. In this study, the deregulation of two miRNAs (rno-miR-18a-5p and rno-miR-345-3p) was described in the ventral prostate VP of old rats born to dams fed with a low protein diet (LPD) (6% protein in the diet) during gestational and lactational periods. Integrative analysis of the (VP) transcriptomic and proteomic data revealed changes in the expression profile of 14 identified predicted targets of these two DE miRNAs, which enriched terms related to post-translational protein modification, metabolism of proteins, protein processing in endoplasmic reticulum, phosphonate and phosphinate metabolism, the calnexin/calreticulin cycle, metabolic pathways, N-glycan trimming in the ER and the calnexin/calreticulin cycle, hedgehog ligand biogenesis, the ER-phagosome pathway, detoxification of reactive oxygen species, antigenprocessing-cross presentation, RAB geranylgeranylation, collagen formation, glutathione metabolism, the metabolism of xenobiotics by cytochrome P450, and platinum drug resistance. RT-qPCR validated the deregulation of the miR-18a-5p/P4HB (prolyl 4-hydroxylase subunit beta) network in the VP of older offspring as well as in the PNT-2 cells transfected with mimic miR-18a-5p. Functional in vitro studies revealed a potential modulation of estrogen receptor α (ESR1) by miR-18a-5p in PNT-2 cells, which was also confirmed in the VP of older offspring. An imbalance of the testosterone/estrogen ratio was also observed in the offspring rats born to dams fed with an LPD. In conclusion, deregulation of the miR-18a-5p/P4HB network can contribute to the developmental origins of prostate cancer in maternally malnourished offspring, highlighting the need for improving maternal healthcare during critical windows of vulnerability early in life.
  • ItemArtigo
    Sperm phylogeny of Characidae (Teleostei, Characiformes)
    (2023-03-01) Mirande, Juan Marcos; Baicere-Silva, Clarianna M.; Santana, Júlio C. O.; Quagio-Grassiotto, Irani [UNESP]; Fundación Miguel Lillo – Unidad Ejecutora Lillo (FML-CONICET); Universidade de Brasília (UnB); Universidade Estadual Paulista (UNESP)
    The phylogeny of the very diverse Neotropical fish family Characidae has been the subject of several recent contributions based on morphological characters, molecular data or both in combined analyses. In cases of conflict between these kinds of data, resolution by combined analyses most often tends to agree with the molecular evidence, given the disproportionate number of characters it contains in comparison with morphological datasets. This happens especially after the advent of massive DNA sequencing methods. In this contribution, we present the most comprehensive set of characters from sperm and spermiogenesis of the Characidae. Since these traits are not expected to be functionally correlated with the general morphology or molecular markers, we consider them a third source of data. We provide a phylogenetic analysis from a combined dataset of seven molecular markers (6444 characters), general morphology (520 characters) and reproductive features (94 characters) coded for 165 species of characiform fishes. Parsimony analyses were done under extended implied weighting under 30 different combinations of weighting schemes and strengths. Most parsimonious trees from two different weighting conditions were selected as representative samples of the obtained topologies, in order to evaluate the performance of the reproductive characters. One of these hypotheses is more conservative regarding the currently accepted phylogenies and the other is the most parsimonious tree that we found as the best correlated with the morphological data. Reproductive characters are shown to be more homoplastic than general morphology and DNA, but provided synapomorphies for 23–24 nodes that had no morphological synapomorphies, justifying their use in phylogenetic analyses. Also, in combination with data from general morphology and considering details of the phylogenetic analysis, they showed to have the potential to challenge well-established hypotheses based on molecular data.
  • ItemLivro
    Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans
    (2015-01-01) Ozouf-Costaz, Catherine; Pisano, Eva; Foresti, Fausto [UNESP]; de Almeida Toledo, Lurdes Foresti; UPMC; University of Genova; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
    Recent advances in fish cytogenetics have enhanced the interest in chromosome analysis in both fundamental (systematics and comparative genomics among fishes and other vertebrate groups) and applied (aquaculture, conservation and response to pollutants, whole genome sequencing of model fish species) research. Although the genomic material, the chro.
  • ItemEditorial
    Preface
    (2015-01-01) Ozouf-Costaz, Catherine; Pisano, Eva; Foresti, Fausto [UNESP]; de Almeida Toledo, Lurdes Foresti; UPMC; University of Genova; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)
  • ItemArtigo
    Transcriptomic insight into the hybridization mechanism of the Tambacu, a hybrid from Colossoma macropomum (Tambaqui) and Piaractus mesopotamicus (Pacu)
    (2023-03-01) Mareco, Edson Assunção; de la Serrana, Daniel Garcia; de Paula, Tassiana Gutierrez [UNESP]; Zanella, Bruna Tereza Thomazini [UNESP]; da Silva Duran, Bruno Oliveira [UNESP]; Salomão, Rondinelle Artur Simões; de Almeida Fantinatti, Bruno Evaristo [UNESP]; de Oliveira, Victor Hugo Garcia; dos Santos, Vander Bruno; Carvalho, Robson Francisco [UNESP]; Dal-Pai-Silva, Maeli [UNESP]; University of Western São Paulo; University of Barcelona; Universidade Estadual Paulista (UNESP); Instituto de Pesca/APTA/SAA
    Interspecific hybrids are highly complex organisms, especially considering aspects related to the organization of genetic material. The diversity of possibilities created by the genetic combination between different species makes it difficult to establish a large-scale analysis methodology. An example of this complexity is Tambacu, an interspecific hybrid of Colossoma macropomum (Tambaqui) and Piaractus mesopotamicus (Pacu). Either genotype represents an essential role in South American aquaculture. However, despite this importance, the genetic information for these genotypes is still highly scarce in specialized databases. Using RNA-Seq analysis, we characterized the transcriptome of white muscle from Pacu, Tambaqui, and their interspecific hybrid (Tambacu). The sequencing process allowed us to obtain a significant number of reads (approximately 53 billion short reads). A total of annotated contigs were 37,285, 96,738, and 158,709 for Pacu, Tambaqui, and Tambacu. After that, we performed a comparative analysis of the transcriptome of the three genotypes, where we evaluated the differential expression (Tambacu vs Pacu = 11,156, and Tambacu vs Tambaqui = 876) profile of the transcript and the degree of similarity between the nucleotide sequences between the genotypes. We assessed the intensity and pattern of expression across genotypes using differential expression information. Clusterization analysis showed a closer relationship between Tambaqui and Tambacu. Furthermore, digital differential expression analysis selected some target genes related to essential cellular processes to evaluate and validate the expression through the RT-qPCR. The RT-qPCR analysis demonstrated significantly (p < 0.05) elevated expression of the mafbx, foxo1a, and rgcc genes in the hybrid compared to the parents. Likewise, we can observe genes significantly more expressed in Pacu (mtco1 and mylpfa) and mtco2 in Tambaqui. Our results showed that the phenotype presented by Tambacu might be associated with changes in the gene expression profile and not necessarily with an increase in gene variability. Thus, the molecular mechanisms underlying these “hybrid effects” may be related to additive and, in some cases, dominant regulatory interactions between parental alleles that act directly on gene regulation in the hybrid transcripts.