Effect of analogues of cationic peptides on dentin mineralization markers in odontoblast-like cells
Nenhuma Miniatura disponível
Data
2019-07-01
Autores
Caiaffa, Karina S. [UNESP]
Basso, Fernanda G. [UNESP]
Santos-Filho, Norival A. [UNESP]
de Souza-Costa, Carlos Alberto [UNESP]
Sakai, Vivien T.
Cilli, Eduardo M. [UNESP]
Duque, Cristiane [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Objectives: To evaluate the effect of analogues of cationic peptides on the viability and the expression of phenotypic and genotypic markers of dentin mineralization in MDPC-23 odontoblast-like cells. Materials and methods: Cells were exposed to serial dilutions of analogues of cationic peptides hBD-3-1CV and KR-12-a5 compared to peptide LL-37 and their viability was assessed by methyltetrazolium assay. Next, peptides (0.78–62.5 μg/mL) were applied on the MDPC-23 cells for evaluating the total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition. Gene expression of mineralization markers (DSPP and DMP-1) was also determined by quantitative PCR. Results: LL-37 and hBD-3-1CV treatment did not affect cellular viability at concentrations below 62.5 μg/mL. KR-12-a5 reduced cell viability above 31.25 μg/mL. TP production was similar for all groups compared with the control group, except by hBD-3-1CV (at 15.62 μg/mL). LL-37 (at 62.5 μg/mL) induced higher ALP activity than control and other experimental groups. LL-37 and hBD-3-1CV, at 62.5 μg/mL and KR-12-a5 at 31.25 μg/mL stimulated the highest deposition of mineralized nodule. Overall, no statistical differences were observed between the groups for DSPP-1 and DMP-1 expressions. Conclusions: LL-37 was the only peptide that induced both ALP activity and mineralized nodules deposition, without affecting cell viability. None of peptides tested induced the expression of DSPP or DMP-1, genes commonly involved in active dentin mineralization.
Descrição
Palavras-chave
Cationic antimicrobial peptides, Cell culture, Cytotoxicity, Endodontics, Polymerase chain reaction
Como citar
Archives of Oral Biology, v. 103, p. 19-25.