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dc.contributor.authorAzevedo, MFC [UNESP]
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.authorRamos, PRR [UNESP]
dc.contributor.authorJim, J [UNESP]
dc.date.accessioned2014-05-20T13:53:59Z
dc.date.available2014-05-20T13:53:59Z
dc.date.issued2003-01-01
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572003000300012
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 26, n. 3, p. 289-294, 2003.
dc.identifier.issn1415-4757
dc.identifier.urihttp://hdl.handle.net/11449/19285
dc.description.abstractSpecimens of Bufo ictericus, Bufo paracnemis and a third type, considered an intermediate subgroup between these species, were cytogenetically studied by conventional Giemsa staining, C-banding and staining of the nucleolus organizer region (NOR). The nuclear DNA content and seroproteins were also analyzed to characterize these species, and verify the possibility of hybridization between them. Karyotypes and cytogenetic markers were essentially equal on the basis of the methods used. The DNA nuclear content found was 6.25 ± 0.30 pg/DNA in Bufo ictericus; 7.57 ± 0.40 pg/DNA in Bufo paracnemis and 7.04 ± 0.29 pg/DNA in the intermediate subgroup. Eletrophoresis of total blood serum in Bufo ictericus, Bufo paracnemis and the intermediate specimens revealed a remarkable difference in the patterns of the protein bands whose molecular weight corresponded to that of albumin. While the parental species presented two different bands, the intermediate form presented 4. However, only three of these bands were seen in each specimen. The results obtained pointed to a high probability for natural hybridization between Bufo ictericus and Bufo paracnemis in the site and specimens studied.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent289-294
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.sourceSciELO
dc.subjectBufoen
dc.subjecthybridizationen
dc.subjectcytogeneticsen
dc.subjectnuclear DNAen
dc.subjectseroproteinsen
dc.titleComparative cytogenetic studies of Bufo ictericus, B. paracnemis (Amphibia, Anura) and an intermediate form in sympatryen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUNESP Instituto de Biociências Departamento de Zoologia
dc.description.affiliationUNESP Instituto de Biociências Departamento de Morfologia
dc.description.affiliationUNESP Instituto de Biociências Departamento de Física e Biofísica
dc.description.affiliationUnespUNESP Instituto de Biociências Departamento de Zoologia
dc.description.affiliationUnespUNESP Instituto de Biociências Departamento de Morfologia
dc.description.affiliationUnespUNESP Instituto de Biociências Departamento de Física e Biofísica
dc.identifier.doi10.1590/S1415-47572003000300012
dc.identifier.scieloS1415-47572003000300012
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatupt
dc.identifier.fileS1415-47572003000300012.pdf
dc.identifier.lattes0804793944846367
dc.identifier.lattes1842465525706144
unesp.author.lattes0804793944846367
unesp.author.lattes1842465525706144
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
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