Ultrastructure of last larval instar fat body cells of Pachycondyla (= Neoponera) villosa (Formicidae : Ponerinae): cytochemical and chemical analysis
Nenhuma Miniatura disponível
Data
2003-07-01
Autores
Zara, F. J.
Caetano, F. H.
Cabrera, ACG
Jaffe, K.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Brill Academic Publishers
Resumo
The ultrastructure of the fat body cells (trophocytes) of the last larval instar of Pachycondyla (= Neoponera) villosa is presented. The cytoplasm is restricted to the cell periphery and to the smaller strips among the vacuoles, protein granules, lipid droplets, and around the nucleus. Cytochemically, the presence of basic amino acids in the protein granules and in the nuclei was observed by using the ethanolic phosphotungstic acid technique (EPTA). The lipid droplets stained for unsaturated lipids. This result was further confirmed by gas chromatography and mass spectrometry, where the unsaturated fatty acids were identified as oleic and linoleic acids together with saturated fatty acids such as palmitic and stearic acid. Carbohydrates (glycogen) were also detected in the fat body. The glycogen is present as beta particles distributed among the lipid droplets and sometimes attached to them.
Descrição
Palavras-chave
chromatography, cytochemistry, fat body, larva, Pachycondyla villosa, ultrastructure
Como citar
Animal Biology. Leiden: Brill Academic Publishers, v. 53, n. 1, p. 1-16, 2003.