Mutagenic, genotoxic and morphotoxic potential of different pesticides in the erythrocytes of Podocnemis expansa neonates
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Data
2020-10-01
Autores
Pereira de Oliveira, Jose Silonardo
Vieira, Lucelia Goncalves
Carvalho, Wanessa Fernandes
Souza, Marcelino Benvindo de
Lima Rodrigues, Aline Sueli de
Simoes, Karina
De Silva, Daniela de Melo
Mendonca, Juliana dos Santos [UNESP]
Luz Hirano, Liria Queiroz
Quagliatto Santos, Andre Luiz
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Despite the damaging effects of pesticides glyphosate (Gly), atrazine (Atra) and fipronil (lip) on different organisms, the mutagenic, genotoxic and morphotoxic potential of testudine erythrocytes in freshwater remains unknown. Thus, the aim of the present study is to assess the toxicological potential of these compounds in Podocnemis expansa (Amazonian turtles) neonates from eggs artificially incubated in substrate at different concentrations of herbicides Gly and Atra and insecticide Pip. Micronucleus test and other nuclear abnormalities, as well as comet assay and morphometric measurements taken of models circulating erythrocytes were used as toxicity biomarkers. Pups exposed to Gly (groups Gly-65 ppb and Gly-6500 ppb) were the ones recording the largest amount of nuclear abnormalities; erythrocytes with multilobulated, notched and displaced nucleus were mostly frequent in groups Atra-2 ppb and Gly-65 ppb. All treatments (Gly-6500 ppb, Atra-2 ppb, Atra-200 ppb, Fip-4 ppb and Fip-400 ppb), except for group Gly-65 ppb, led to decreased erythrocyte area, increased nuclear area: erythrocyte area ratio, as well as to decreased erythrocyte and erythrocyte nuclei circularity, which highlights the clear effect on the size and shape of these cells. On the other hand, the cornet assay did not evidence any genotoxic effect caused by the assessed pesticides. This is a pioneer study on the mutagenic and morphotoxic potential of pesticides in P. expansa eclodides exposed in ovo to Gly, Atra and Fip; therefore, it is an insight on how these compounds can affect the health of these animals. (C) 2020 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Pesticides, Ecotoxicology, Mutagenicity, Genotoxicity, Morphotoxicity
Como citar
Science Of The Total Environment. Amsterdam: Elsevier, v. 737, 9 p., 2020.