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dc.contributor.authorRocha, Thalita
dc.contributor.authorde Souza, Bibiana M. [UNESP]
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorda Cruz-Hoefling, Maria Alice
dc.contributor.authorHarris, John Buchanan
dc.date.accessioned2014-05-20T13:55:28Z
dc.date.available2014-05-20T13:55:28Z
dc.date.issued2009-10-01
dc.identifierhttp://dx.doi.org/10.1007/s00418-009-0607-z
dc.identifier.citationHistochemistry and Cell Biology. New York: Springer, v. 132, n. 4, p. 395-404, 2009.
dc.identifier.issn0948-6143
dc.identifier.urihttp://hdl.handle.net/11449/19849
dc.description.abstractPolybia-MPII (INWLKLGKMVIDAL-NH(2)), a mastoparan isolated from the crude venom of the swarming wasp Polybia paulista, was injected into the left hind limb of Swiss white mice. Between 3 h and 21 days later the mice were killed and the soleus muscles from both hind limbs were removed. Sections of the muscles were made for transmission electron microscopy and immunocytochemistry. Transmission electron microscopy showed that both the volume fraction occupied by synaptic vesicles and synaptic vesicle density was greatly reduced after exposure to Polybia-MPII, although there was no significant structural damage to the plasma membrane of the terminal boutons and mitochondria were indistinguishable from those in normal, control boutons. Immunocytochemistry revealed that in control muscles 99% of motor end plates identified by the positive labelling of acetylcholine receptors by TRITC-alpha-bungarotoxin co-labelled with anti-synaptophysin antibody, but this figure fell by 30% in muscles exposed to the toxin. These changes were transient. They were maximal at 6 h and fully reversed by 3 days. At no time was axonal labelling with anti-neurofilament antibodies affected by exposure to Polybia-MPII. We conclude that mastoparan Polybia-MPII is a minor neurotoxin and suggest that its neurotoxic activity is unlikely to be of clinical significance.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent395-404
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofHistochemistry and Cell Biology
dc.sourceWeb of Science
dc.subjectWaspen
dc.subjectPolybia paulistaen
dc.subjectSynaptic vesiclesen
dc.subjectSynaptophysinen
dc.subjectNeurofilamentsen
dc.titleThe neurotoxicological effects of mastoparan Polybia-MPII at the murine neuromuscular junction: an ultrastructural and immunocytochemical studyen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
dc.contributor.institutionUniversity of Newcastle
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniv Newcastle, Med Toxicol Ctr, Inst Neurosci, Sch Med, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
dc.description.affiliationUniv Estadual Campinas, Dept Histol & Embryol, Inst Biol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Biol, Inst Biosci,Ctr Studies Social Insects, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Biol, Inst Biosci,Ctr Studies Social Insects, BR-13506900 Rio Claro, SP, Brazil
dc.identifier.doi10.1007/s00418-009-0607-z
dc.identifier.wosWOS:000270171400003
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdCNPq: 210284/2006-4
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
dc.identifier.lattes2901888624506535
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-2731-9586[1]
unesp.author.orcid0000-0002-7363-8211[3]
dc.relation.ispartofjcr2.164
dc.relation.ispartofsjr1,044
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