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dc.contributor.authorSemensato, Juliana [UNESP]
dc.contributor.authorFernandes, Júlio Cesar
dc.contributor.authorBenderdour, Mohamed
dc.contributor.authorde Oliveira Tiera, Vera Aparecida [UNESP]
dc.contributor.authorLima, Aline Margarete Furuyama [UNESP]
dc.contributor.authorTiera, Marcio José [UNESP]
dc.identifier.citationMacromolecular Research, v. 28, n. 2, p. 126-135, 2020.
dc.description.abstractThe improvement of biological properties of polycations is a fundamental step to overcome their limitations as non-viral gene carriers. This work studied the effect of phosphorylcholine (PC) groups on the physicochemical properties of poly(L-lysine) (PLL)/pDNA nanoparticles. Phosphorylcholine-grafted PLL derivatives (PLL-PC) containing increasing proportions of PC were obtained by the reductive amination reaction with phosphoryl glyceraldehyde and characterized by 1H NMR, FTIR, and GPC measurements. The PLL-PC derivatives were used to prepare polyplexes with pDNA and their properties were evaluated by fluorescence, gel electrophoresis and dynamic light scattering (DLS) measurements. The PLL-PC derivatives were able to interact with pDNA at low N/P ratios in physiological pH to form stable polyplexes having lower zeta potentials, as evidenced by the gel electrophoresis and zeta potentials measurements. A degree of grafting of 10% increased the in vitro transfection efficiency of PLL and a degree of 20 mol% of PC groups provided colloidal stability in physiological saline solution at neutral pH. Overall, the PC-PLL derivatives exhibited improved physicochemical properties and have significant potential for further studies as non-viral gene transfer agents. [Figure not available: see fulltext.].en
dc.relation.ispartofMacromolecular Research
dc.subjectnon-viral gene therapy
dc.titleTuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pHen
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversité de Montréal
dc.description.affiliationDepartamento de Química e Ciências Ambientáis UNESP-Universidade Estadual Paulista
dc.description.affiliationOrthopedic Research Laboratory Hôpital du Sacré-Coeur de Montréal Université de Montréal
dc.description.affiliationUnespDepartamento de Química e Ciências Ambientáis UNESP-Universidade Estadual Paulista
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